采用石蜡组织切片和扫描电镜法,对齐口裂腹鱼卵巢发育进行了组织学研究。
By using the routine method of wax section and scanning electron microscope, the development of the ovary of Schizothorax prenanti was studied.
然而,假阴性率高、结果不稳定等因素严重阻碍着该技术的广泛应用,在石蜡组织中尤其如此。
However, the major obstacle to use technique as a routing is its high false negative rates and inconstant results, especially in paraffin-embedded tissues.
另将191例肝癌石蜡组织制成芯片观察ny -ESO - 1蛋白在肝癌中的分布和表达。
NY-ESO-1 expression and its distribution were further studied by immunohistochemistry in a tissue array contained 191 cases of HCC.
方法根据嗜神经侵袭的病理现象,将32例人涎腺腺样囊性癌(ACC)石蜡组织划分为未见神经侵袭组和存在神经侵袭组。
Methods 32 human salivary ACC paraffin-embedded tissue samples were divided into two groups according to the pathological presence of perineural invasion.
结果石蜡组织切片免疫组化反应预处理,采用中温水浴抗原修复法,其阳性表达率为5 2 .6% ,脱片率为2 .0 % ;
Results Prerteat organization chip, using the middle temperature water bath antigen repairing method, the positive expressing rate is 52.6% and the unfalling rate is 2.0%;
假体周围组织的典型地区分别嵌入常规切片和苏木精和伊红染色石蜡块。
Representative pieces of periprosthetic tissue were embedded in paraffin blocks for routine sectioning and staining with hematoxylin and eosin.
全部组织均经10%福尔马林固定,石蜡包埋,术前均未行放疗,化疗或者其他生物治疗。
The tissues were fixed in 10% formalin and embedded in paraffin. No patients had received preoperative chemotherapy , radiation therapy or other biological therapy.
组织,切片,病人,肝硬化,肝石蜡切片。
Tissue, Section, Human Disease, Liver Cirrhosis, Liver Paraffin.
方法组织培养和石蜡切片。
利用组织透明法、石蜡制片法和薄切片法比较研究了樟科5属13种植物叶片中油细胞和粘液细胞的分布和结构。
The oil cells and mucilage cells in the leaves of 13 species in 5 genera (Lauraceae) were comparatively studied with the methods of tissue clearing, paraffin sectioning and semi-thick sectioning.
报道人体及动物组织石蜡切片方法的全面革新技术。
This article reports the all around innovation technology of paraffin slice method for human and animal tissues.
样品为石蜡包埋的肺肿瘤组织块,平均保存13.4年。
Cancer tissues were embedded in paraffin and stored for 13.4 years in average.
石蜡制片是观察植物组织构造常用的制片方法之一。
Paraffin sectioning is a common sectioning method for observing the plant's tissue structure.
本研究还发现,检测石蜡切片肝组织内抗原时,阻断内源性生物素并不是一个必不可少的步骤。
In this study, it also has been shown that blocking endogenous biotin is not a necessary procedure in detecting tissue antigen on paraffin embeded section.
搜集裸鼠各脏器组织,10%缓冲福尔·马林固定,常规石蜡包埋,HE染色,镜下观察形态学改变。
Gathered viscus tissues of nude mouse, fixed with 10% dampen formaldehyde solution, paraffin embed, routine he stained and morphology observed by microscope.
结论石蜡包埋组织中提取RNA方法稳定,可为疾病相关基因检测提供有效手段,在科研和临床方面有广阔的应用前景。
Conclusions The way to extract RNA from paraffin-embedded tissue is stable and feasible, and it is a good method for disease-associated gene detection.
目的探讨固定剂及固定时间对石蜡包埋rna病毒感染组织中RNA降解及标本病理形态学的影响。
Objective To study the effect of fixatives and fixed_time on the morphology and detectability of viral RNA from paraffine_embedded tissues by RT_PCR.
此外,本文还对不同组织的石蜡包埋块电镜制样的效果进行了比较和讨论。
The effects of the method in various tissues were compared and discussed briefly in the article.
组织,切片,病人,肝硬化,脾石蜡切片。
Tissue, Section, Human Disease, Liver Cirrhosis, Spleen Paraffin.
方法应用免疫组化法对101例大肠癌组织石蜡标本进行MAGE-3抗原表达测定。
Method Immunohistochemical technique for MAGE-3 antigen was performed in paraffin embedded sections of 101 CRC specimens.
方法取11~36周人胎肺组织常规石蜡包埋切片,HE染色观察胚肺各个发育阶段的成熟度,免疫组化检测SP-D时相性表达特征。
Methods Human fetal lung tissues from 11- to 36-week-old fetuses were assayed for lung maturity with HE staining and SP-D expression by means of immunohistochemistry.
由于甲醛介导的DNA损伤和石蜡对DNA提取的阻碍作用,使得常规DNA提取方法很难从福尔马林固定石蜡包埋组织(FFPET)中获取高质量的DNA。
Owing to the DNA degradation induced by formalin and the obstruction of paraffin to DNA extraction, it is difficult to recover high-quality DNA from Formalin fixed and paraffin embedded tissue(FFPET).
石蜡和冰冻组织标本结果一致。
Similar results were obtained in paraffin embedded and frozen sections.
方法应用大脑锥体细胞组织块浸银染色,石蜡包埋切片显微镜观察大脑锥体细胞。
Methods With pyramidal cell tissues stained in silver nitrate, brain pyramidal cells were displayed by paraffin section method.
通过优化实验条件,可以在石蜡包埋组织上成功地进行FISH实验。
By the optimization of experimental conditions, FISH can be successfully performed on paraffin-embedded tissue.
目的研究石蜡包埋胰腺癌组织中DPC4基因的改变。
Objective To examine the alteration and significance of the DPC4 gene in paraffin-embedded tissues of pancreatic carcinomas.
提取120例拟诊为结核病的石蜡包埋组织样本dna,经酚-氯仿-异戊醇纯化后用PCR技术扩增结核分枝杆菌特异序列is6110片段。
PCR technique was used to detect the IS6110 specific insertion sequence DNA of Mycobacterium tuberculosis complex in the 120 paraffin-embedded tissues.
从石蜡包埋组织中提取DNA,是进一步研究抑癌基因的基础。
It is the foundation to further research anti-oncogene to extract DNA from paraffin embed constitution.
应用流式细胞术对5例先天性中胚层肾瘤、96例肾母细胞瘤和27例正常肾石蜡包埋组织进行了DNA含量和增殖活性分析。
The DNA content and cell cycle in paraffin-embedded sections collected from 5 cases of congenital mesoblastic nephroma. 96 cases of nephroblastoma and 27 normal kidneys were studied by flow cytometry.
结论应用RT-PCR或RQ-PCR方法在石蜡包埋组织中检测目的基因的表达是可行的。
Conclusions Detection of target gene expression in paraffin-embedded tissues is feasible by RT-PCR or RQ-PCR.
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