从石蜡包埋组织中提取DNA，是进一步研究抑癌基因的基础。
It is the foundation to further research anti-oncogene to extract DNA from paraffin embed constitution.
通过优化实验条件，可以在石蜡包埋组织上成功地进行FISH实验。
By the optimization of experimental conditions, FISH can be successfully performed on paraffin-embedded tissue.
结论应用RT-PCR或RQ-PCR方法在石蜡包埋组织中检测目的基因的表达是可行的。
Conclusions Detection of target gene expression in paraffin-embedded tissues is feasible by RT-PCR or RQ-PCR.
结论：合适的福尔·马林固定石蜡包埋组织dna提取方法可应用PCR进行DNA水平的相应的研究。
Conclusion: Appropriate DNA extract methods for formalin fixed and paraffin embedded tissues may benifit PCR.
目的探讨单管巢式聚合酶链反应(SNPCR)检测石蜡包埋组织结核分支杆菌dna的特异性和敏感性。
Objective To study the sensitivity and specificity of single tube nested polymerase chain reaction (SN PCR) technique in detecting Mycobacterium tuberculosis DNA in paraffin embedded tissues.
结果100%的新鲜组织和24.1%的石蜡包埋组织至少可以得到甲基化和非甲基化产物中的一种产物。
Results 100 % of frozen tissues and 24.1% of paraffin-embedded tissues could get either methylated production or unmethylated production.
方法：实验选取山西医科大学第二医院病理科2000 ~ 2005年间经手术切除的GCT石蜡包埋组织30例。
Methods: we select 30 GCT paraffin samples which had been excised in surgery between 2000 and 2005 from the pathology department of the second hospital of Shanxi medical college.
结论石蜡包埋组织中提取RNA方法稳定，可为疾病相关基因检测提供有效手段，在科研和临床方面有广阔的应用前景。
Conclusions The way to extract RNA from paraffin-embedded tissue is stable and feasible, and it is a good method for disease-associated gene detection.
应用流式细胞术对5例先天性中胚层肾瘤、96例肾母细胞瘤和27例正常肾石蜡包埋组织进行了DNA含量和增殖活性分析。
The DNA content and cell cycle in paraffin-embedded sections collected from 5 cases of congenital mesoblastic nephroma. 96 cases of nephroblastoma and 27 normal kidneys were studied by flow cytometry.
提取120例拟诊为结核病的石蜡包埋组织样本dna，经酚-氯仿-异戊醇纯化后用PCR技术扩增结核分枝杆菌特异序列is6110片段。
PCR technique was used to detect the IS6110 specific insertion sequence DNA of Mycobacterium tuberculosis complex in the 120 paraffin-embedded tissues.
由于甲醛介导的DNA损伤和石蜡对DNA提取的阻碍作用，使得常规DNA提取方法很难从福尔马林固定石蜡包埋组织（FFPET）中获取高质量的DNA。
Owing to the DNA degradation induced by formalin and the obstruction of paraffin to DNA extraction, it is difficult to recover high-quality DNA from Formalin fixed and paraffin embedded tissue(FFPET).
全部组织均经10%福尔马林固定，石蜡包埋，术前均未行放疗，化疗或者其他生物治疗。
The tissues were fixed in 10% formalin and embedded in paraffin. No patients had received preoperative chemotherapy , radiation therapy or other biological therapy.
样品为石蜡包埋的肺肿瘤组织块，平均保存13.4年。
Cancer tissues were embedded in paraffin and stored for 13.4 years in average.
方法应用大脑锥体细胞组织块浸银染色，石蜡包埋切片显微镜观察大脑锥体细胞。
Methods With pyramidal cell tissues stained in silver nitrate, brain pyramidal cells were displayed by paraffin section method.
搜集裸鼠各脏器组织，10%缓冲福尔·马林固定，常规石蜡包埋，HE染色，镜下观察形态学改变。
Gathered viscus tissues of nude mouse, fixed with 10% dampen formaldehyde solution, paraffin embed, routine he stained and morphology observed by microscope.
目的探讨固定剂及固定时间对石蜡包埋rna病毒感染组织中RNA降解及标本病理形态学的影响。
Objective To study the effect of fixatives and fixed_time on the morphology and detectability of viral RNA from paraffine_embedded tissues by RT_PCR.
方法福尔·马林固定、石蜡包埋40例肉瘤标本进行HE和TSG101免疫组织化学染色，观察tsg101的表达情况。
Methods 40 formalin fixed and paraffin embeded sarcoma tissues were stained by he and immunohistochemistry to observe the expression of TSG101.
此外，本文还对不同组织的石蜡包埋块电镜制样的效果进行了比较和讨论。
The effects of the method in various tissues were compared and discussed briefly in the article.
方法取11~36周人胎肺组织常规石蜡包埋切片，HE染色观察胚肺各个发育阶段的成熟度，免疫组化检测SP-D时相性表达特征。
Methods Human fetal lung tissues from 11- to 36-week-old fetuses were assayed for lung maturity with HE staining and SP-D expression by means of immunohistochemistry.
方法：收集健康新鲜的前磨牙，按年龄分组，石蜡包埋切片，免疫组织化学与图像定量分析。
Methods: Fresh health premolars were collected, divided into some groups according to age, embedded in paraffin and cut into slices.
大脑组织形态学观察：大鼠脑组织用甲醛固定24小时，石蜡包埋，制作病理切片，HE染色。
Observation of brain tissues morphology: Rats brain tissue with formaldehyde 24 hours, embedded in paraffin, making pathological section, HE stain.
目的研究石蜡包埋胰腺癌组织中DPC4基因的改变。
Objective To examine the alteration and significance of the DPC4 gene in paraffin-embedded tissues of pancreatic carcinomas.
试验方法为大体解剖观察与拍照，切取组织块经石蜡包埋、切片、H。
First, digestive gland was observed, measured and taken a photograph. Then, tissue pieces were taken to paraffin imbedding, and cut, H.
采血后取肝组织，常规石蜡包埋，切片做HE染色观察肝组织的病理变化，采用TUNEL法检测肝细胞的凋亡。
Meanwhile, the pathological changes of liver was observed under light microscope after he staining, and hepatocyte apoptosis was detected by the TUNEL method.
并取肝左叶于10％中性甲醛溶液中固定，石蜡包埋，作组织形态学观察。
Left lobe of liver was fixed in 10% formaldehyde, than embedelinged in paraffin, at last observed histological change with optical microscope.
搜集裸鼠各脏器组织，0%缓冲福尔·马林固定，常规石蜡包埋，HE染色，镜下观察形态学改变。
Gathered viscus tissues of nude mouse, fixed with 0% dampen formaldehyde solution, paraffin embed, routine he stained and morphology observed by microscope.
从患可手术的乳腺癌患者中获得93例福尔·马林固定石蜡包埋(FFPE)的组织，来验证RASSF1A甲基化的预后重要性。
The prognostic significance of RASSF1A methylation was validated in 93 formalin fixed paraffin-embedded (FFPE) tissues obtained from patients with operable breast cancer.
方法石蜡包埋切片，应用免疫组织化学SP法及麦克奥迪真彩色病理分析图像分析系统，对TTF-1表达强度的阳性单位（PU）进行定量分析。
Methods The TTF-1 expression intensity (in positive units, PU) in paraffin-embedded tissue blocks was determined using immunohistochemical SP method in combination with Motic Images System.
解剖取胰腺，采用石蜡包埋、HE染色，做组织切片观察。
The paraffin sections of pancreas, stained with he, were made into pathological sections for observation.
方法：采用免疫组化方法检测64例脑胶质瘤和10例正常脑组织石蜡包埋标本中CENP-F的表达。
Methods: Immunohistochemistry was used to detect the expression of CENP- F in 64 cases of brain glioma and 10 cases of normal brain tissues by paraffin- embedded.
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