用免疫组化LSAB的方法对石蜡包埋的组织切片进行MAGE抗原的检测。
Method Using LSAB immunohistochemical technique to detect the expression of MAGE 3 antigen in formalin fixed, paraffin embedded sections.
目的探讨固定剂及固定时间对石蜡包埋rna病毒感染组织中RNA降解及标本病理形态学的影响。
Objective To study the effect of fixatives and fixed_time on the morphology and detectability of viral RNA from paraffine_embedded tissues by RT_PCR.
此外,本文还对不同组织的石蜡包埋块电镜制样的效果进行了比较和讨论。
The effects of the method in various tissues were compared and discussed briefly in the article.
方法:收集健康新鲜的前磨牙,按年龄分组,石蜡包埋切片,免疫组织化学与图像定量分析。
Methods: Fresh health premolars were collected, divided into some groups according to age, embedded in paraffin and cut into slices.
结论应用RT-PCR或RQ-PCR方法在石蜡包埋组织中检测目的基因的表达是可行的。
Conclusions Detection of target gene expression in paraffin-embedded tissues is feasible by RT-PCR or RQ-PCR.
从石蜡包埋组织中提取DNA,是进一步研究抑癌基因的基础。
It is the foundation to further research anti-oncogene to extract DNA from paraffin embed constitution.
由于甲醛介导的DNA损伤和石蜡对DNA提取的阻碍作用,使得常规DNA提取方法很难从福尔马林固定石蜡包埋组织(FFPET)中获取高质量的DNA。
Owing to the DNA degradation induced by formalin and the obstruction of paraffin to DNA extraction, it is difficult to recover high-quality DNA from Formalin fixed and paraffin embedded tissue(FFPET).
样品为石蜡包埋的肺肿瘤组织块,平均保存13.4年。
Cancer tissues were embedded in paraffin and stored for 13.4 years in average.
方法:实验选取山西医科大学第二医院病理科2000 ~ 2005年间经手术切除的GCT石蜡包埋组织30例。
Methods: we select 30 GCT paraffin samples which had been excised in surgery between 2000 and 2005 from the pathology department of the second hospital of Shanxi medical college.
结论石蜡包埋组织中提取RNA方法稳定,可为疾病相关基因检测提供有效手段,在科研和临床方面有广阔的应用前景。
Conclusions The way to extract RNA from paraffin-embedded tissue is stable and feasible, and it is a good method for disease-associated gene detection.
方法福尔·马林固定、石蜡包埋40例肉瘤标本进行HE和TSG101免疫组织化学染色,观察tsg101的表达情况。
Methods 40 formalin fixed and paraffin embeded sarcoma tissues were stained by he and immunohistochemistry to observe the expression of TSG101.
采血后取肝组织,常规石蜡包埋,切片做HE染色观察肝组织的病理变化,采用TUNEL法检测肝细胞的凋亡。
Meanwhile, the pathological changes of liver was observed under light microscope after he staining, and hepatocyte apoptosis was detected by the TUNEL method.
方法取11~36周人胎肺组织常规石蜡包埋切片,HE染色观察胚肺各个发育阶段的成熟度,免疫组化检测SP-D时相性表达特征。
Methods Human fetal lung tissues from 11- to 36-week-old fetuses were assayed for lung maturity with HE staining and SP-D expression by means of immunohistochemistry.
目的研究石蜡包埋胰腺癌组织中DPC4基因的改变。
Objective To examine the alteration and significance of the DPC4 gene in paraffin-embedded tissues of pancreatic carcinomas.
提取120例拟诊为结核病的石蜡包埋组织样本dna,经酚-氯仿-异戊醇纯化后用PCR技术扩增结核分枝杆菌特异序列is6110片段。
PCR technique was used to detect the IS6110 specific insertion sequence DNA of Mycobacterium tuberculosis complex in the 120 paraffin-embedded tissues.
方法石蜡包埋切片,应用免疫组织化学SP法及麦克奥迪真彩色病理分析图像分析系统,对TTF-1表达强度的阳性单位(PU)进行定量分析。
Methods The TTF-1 expression intensity (in positive units, PU) in paraffin-embedded tissue blocks was determined using immunohistochemical SP method in combination with Motic Images System.
结论:合适的福尔·马林固定石蜡包埋组织dna提取方法可应用PCR进行DNA水平的相应的研究。
Conclusion: Appropriate DNA extract methods for formalin fixed and paraffin embedded tissues may benifit PCR.
方法:采用免疫组化方法检测64例脑胶质瘤和10例正常脑组织石蜡包埋标本中CENP-F的表达。
Methods: Immunohistochemistry was used to detect the expression of CENP- F in 64 cases of brain glioma and 10 cases of normal brain tissues by paraffin- embedded.
在对照肽(左)或抗原特异性多肽(右)存在的条件下使用FAk抗体对石蜡包埋的人肺癌组织进行免疫组化分析。
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using FAK Antibody in thre presence of control peptide (left) or antigen-specific peptide (right).
目的探讨单管巢式聚合酶链反应(SNPCR)检测石蜡包埋组织结核分支杆菌dna的特异性和敏感性。
Objective To study the sensitivity and specificity of single tube nested polymerase chain reaction (SN PCR) technique in detecting Mycobacterium tuberculosis DNA in paraffin embedded tissues.
免疫组织化学方法检测石蜡包埋的人结肠癌组织,使用的抗体为53bp1。左图是对照组,右图是抗原特异性肽段组。
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using 53bp1 Antibody in the presence of control peptide (left) or antigen-specific peptide (right).
免疫组织化学方法检测石蜡包埋的人肾细胞癌组织,使用的抗体为53bp1。
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma, using 53bp1 Antibody.
植入物石蜡包埋,组织学切片观察丝素。壳聚糖中颌下腺细胞的生长状况。
Scanning electron microscope was used to observe the growth behavior submandibular gland cells on SFCS scaffolds.
免疫组织化学方法检测石蜡包埋的人乳腺癌组织,图示为核定位。使用的抗体为53bp1。
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear localization, using 53bp1 Antibody.
结果100%的新鲜组织和24.1%的石蜡包埋组织至少可以得到甲基化和非甲基化产物中的一种产物。
Results 100 % of frozen tissues and 24.1% of paraffin-embedded tissues could get either methylated production or unmethylated production.
从患可手术的乳腺癌患者中获得93例福尔·马林固定石蜡包埋(FFPE)的组织,来验证RASSF1A甲基化的预后重要性。
The prognostic significance of RASSF1A methylation was validated in 93 formalin fixed paraffin-embedded (FFPE) tissues obtained from patients with operable breast cancer.
免疫组织化学染色分析石蜡包埋人乳腺癌组织。在对照多肽(左图)或抗原特异性封闭多肽(右图)的存在下所用抗体为c - RelAntibody。
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using c-Rel Antibody in the presence of control peptide (left) or antigen-specific peptide (right).
在对照肽段(左)和抗原特异性肽段(右)存在的前提下,使用Wee1 (D10D2)RabbitmAb对石蜡包埋的人结肠癌组织进行免疫组化分析。
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Wee1 (D10D2) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
在对照肽段(左)和抗原特异性肽段(右)存在的前提下,使用Wee1 (D10D2)RabbitmAb对石蜡包埋的人结肠癌组织进行免疫组化分析。
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Wee1 (D10D2) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
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