目的：以CHO - K1细胞为宿主基因瞬时转染条件的优化。
Objective: To optimize the condition of gene transient transfection in CHO-K1 cell.
瞬时转染TFAR19正义基因后，TF-1细胞去细胞因子后凋亡速度增加。
After transient transinfection of TFAR19, the apoptosis of TF 1 cell is enhanced.
瞬时转染人胚胎肾细胞293t，用荧光素酶报告基因检测这两种剪切形式的转录活性。
The transcriptional activity of the two splicing variants was detected with a luciferase reporter in transient transfections of human embryonic kidney293T cells.
用其体外转染地鼠肾细胞BHK-21，经间接免疫荧光法（IFA）检测，证实了JEV-E的瞬时表达。
The transient expression of JEV E in BHK 21 cells was detected by indirect immunofluorescence assay(IFA) with a monoclonal antibody 2H4 against JEV E.
方法细胞瞬时和稳定转染及氯霉素酰基转移酶和芳香族化酶活性测定。
Methods Transient and stable transfection experiments in cells and assays of chloramphenicol acetyltransferase activity and aromatase activity were used.
方法细胞瞬时和稳定转染及氯霉素酰基转移酶和芳香族化酶活性测定。
Methods Transient and stable transfection experiments in cells and assays of chloramphenicol acetyltransferase activity and aromatase activity were used.
应用推荐