目的克隆激活转录因子(atf)5，构建其真核表达载体，观察其在细胞中的表达定位。
Objective to clone the gene activating transcription factor 5 (ATF5), construct the expression plasmid and detect the localization of ATF5 in cultivated cells.
其中M区高度保守，它编码真核生物转录因子的DNA结合域，具有DNA结合和二聚体形成的作用。
The M domain, which encodes the DNA-binding domain and plays the roles of both binding DNA and forming dimmers, is the most conserved region.
其中M区高度保守，它编码真核生物转录因子的DNA结合域，具有DNA结合和二聚体形成的作用。
The M domain, which encodes the DNA-binding sequence and plays the roles of both binding DNA and forming dimmers, is the most conserved region.
真核生物中热激蛋白基因的表达受热激转录因子的调节。
The expression of heat shock protein genes in eukaryotes is regulated by heat shock transcription factor (HSF).
锌指蛋白是一类广泛存在于原核和真核生物体中的重要转录调控因子，在动植物的生长发育过程中均起着重要作用。
The family of zinc finger proteins is an important member of transcription factors which exists widely in prokaryote and eukaryote.
锌指蛋白是一类广泛存在于原核和真核生物体中的重要转录调控因子，在动植物的生长发育过程中均起着重要作用。
The family of zinc finger proteins is an important member of transcription factors which exists widely in prokaryote and eukaryote.
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