目的从人外周血淋巴细胞中克隆出cd 81基因，构建真核表达质粒，并在COS - 7细胞中进行表达。
Aim to clone human CD81 gene from peripheral blood lymphocytes, and construct its eukaryotic expression vector, and then express it in COS-7 cells.
构建小鼠il -4截短型基因真核表达质粒，表达小鼠il - 4受体拮抗体蛋白。
To clone mouse interleukin 4 (mIL-4) truncated gene, construct its eukaryotic expression plasmid pFB-mIL4 and express the truncated protein (murine IL-4 receptor antagonist).
目的构建癌胚抗原（CEA）与热休克蛋白70（HSP70）的真核双表达质粒，并检测其在体外的表达。
Objective To construct the eukaryotic coexpression plasmid encoding carcinoembryonic antigen(CEA) and heat shock proteins 70 (HSP70), then detect the expression of the plasmid in vitro.
序言真核表达质粒的构建和转染是目前医学研究中常用的分子生物学手段。
Introduction The construction and transfection of eukaryotic expression plasmid are commonly molecular biology method used in medical research.
结论成功构建了重组人FHIT真核表达质粒。
Conlusion The human FHIT recombinant eukaryotic expression plasmid was constructed successfully.
目的克隆人促甲状腺激素受体胞外段基因，构建重组真核表达质粒，获得具有免疫学活性的纯化重组蛋白。
Objective to clone and construct the plasmid containing human thyroid stimulating hormone receptor (TSHR) gene ectodomain, and then identify the immunoreactivity of the purified recombinant protein.
结论：成功地构建杜氏利什曼原虫无鞭毛体蛋白基因的真核表达重组质粒，并且该基因在NIH3T3细胞中获得了稳定表达。
CONCLUSION: a recombinant eukaryotic expression plasmid of amastin gene of Leishmania Donovani was successfully constructed, and can be expressed stably in the NIH3T3 cells.
结论：成功地构建杜氏利什曼原虫无鞭毛体蛋白基因的真核表达重组质粒，并且该基因在NIH3T3细胞中获得了稳定表达。
CONCLUSION: a recombinant eukaryotic expression plasmid of amastin gene of Leishmania Donovani was successfully constructed, and can be expressed stably in the NIH3T3 cells.
应用推荐