结论成功构建了重组人FHIT真核表达质粒。
Conlusion The human FHIT recombinant eukaryotic expression plasmid was constructed successfully.
然后经真核表达质粒-脂质体介导，导入C2C 12细胞系。
After mediated by eukaryotic expression plasmid-liposome, the fused protein was introduced into C2C12 cell system.
序言真核表达质粒的构建和转染是目前医学研究中常用的分子生物学手段。
Introduction The construction and transfection of eukaryotic expression plasmid are commonly molecular biology method used in medical research.
该腺病毒和真核表达质粒在肿瘤治疗药物、放射治疗增敏的制备中具有独特的实用价值。
The adenoviruses and the eukaryotic expression plasmid have unique practicality in preparing tumor treating medicines and radiotherapy hypersensitivity.
构建小鼠il -4截短型基因真核表达质粒，表达小鼠il - 4受体拮抗体蛋白。
To clone mouse interleukin 4 (mIL-4) truncated gene, construct its eukaryotic expression plasmid pFB-mIL4 and express the truncated protein (murine IL-4 receptor antagonist).
目的克隆人促甲状腺激素受体胞外段基因，构建重组真核表达质粒，获得具有免疫学活性的纯化重组蛋白。
Objective to clone and construct the plasmid containing human thyroid stimulating hormone receptor (TSHR) gene ectodomain, and then identify the immunoreactivity of the purified recombinant protein.
目的从人外周血淋巴细胞中克隆出cd 81基因，构建真核表达质粒，并在COS - 7细胞中进行表达。
Aim to clone human CD81 gene from peripheral blood lymphocytes, and construct its eukaryotic expression vector, and then express it in COS-7 cells.
这种基因调控模式不仅限于真核生物，细菌利用小分子rna，尤其是那些从CRISPR位点沉默的噬菌体，转座子和质粒表达。
This mode of gene regulation is not restricted to eukaryotes; bacteria utilize small RNAs, notably those made from CRISPR loci that silence the expression of bacteriophages, transposons and plasmids.
目的构建癌胚抗原（CEA）与热休克蛋白70（HSP70）的真核双表达质粒，并检测其在体外的表达。
Objective To construct the eukaryotic coexpression plasmid encoding carcinoembryonic antigen(CEA) and heat shock proteins 70 (HSP70), then detect the expression of the plasmid in vitro.
酵母表达系统是在酿酒酵母质粒的发现和酵母转化技术的成熟基础上建立起来的真核生物表达系统。
Yeast expression systems are based on the discover of plasmid in Saccharomyces cere- visiae and success of yeast transformation.
结论：成功地构建杜氏利什曼原虫无鞭毛体蛋白基因的真核表达重组质粒，并且该基因在NIH3T3细胞中获得了稳定表达。
CONCLUSION: a recombinant eukaryotic expression plasmid of amastin gene of Leishmania Donovani was successfully constructed, and can be expressed stably in the NIH3T3 cells.
结论：成功地构建杜氏利什曼原虫无鞭毛体蛋白基因的真核表达重组质粒，并且该基因在NIH3T3细胞中获得了稳定表达。
CONCLUSION: a recombinant eukaryotic expression plasmid of amastin gene of Leishmania Donovani was successfully constructed, and can be expressed stably in the NIH3T3 cells.
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