本研究旨在构建具有猪 MSTN 前肽基因定点突变的真核表达载体。
This research intended to construct a eukaryotic expression vector with a site-directed mutation of porcine MSTN propeptide gene.
结论成功构建了重组人FHIT真核表达质粒。
Conlusion The human FHIT recombinant eukaryotic expression plasmid was constructed successfully.
目的：构建高免疫原性的抑制素真核表达载体。
Objective: to construct inhibin expression vector with high immunogenicity.
MCHR2；真核表达载体；转染；基因表达。
MCHR2; eukaryotic expression vector; transfection; gene expression.
目的：研究差异表达新基因BQ135232的真核表达。
Objective: To study the expression of differential expression novel gene BQ135232 obtained by SSH.
然后经真核表达质粒-脂质体介导，导入C2C 12细胞系。
After mediated by eukaryotic expression plasmid-liposome, the fused protein was introduced into C2C12 cell system.
结果酶切和测序证实PTEN基因克隆和真核表达载体构建成功。
Results Restriction enzyme analysis and DNA sequence analysis showed that PTEN gene was cloned and the eukaryotic expression vector was constructed successfully.
目的构建水痘-带状疱疹病毒(VZV)糖蛋白e的真核表达载体。
Objective to construct the eukaryotic expression vector of varicella-zoster virus (VZV) glycoprotein e.
序言真核表达质粒的构建和转染是目前医学研究中常用的分子生物学手段。
Introduction The construction and transfection of eukaryotic expression plasmid are commonly molecular biology method used in medical research.
目的构建大鼠il - 10真核表达载体并在大鼠软骨细胞中进行表达。
Results rat IL-10 eukaryotic expression vector had been constructed successfully, and had been transfected into rat chondrocyte.
构建GGT1重组真核表达载体，观察GGT1在COS7细胞中的定位。
To construct the eukaryotic expression vector of GGT1 and detect its localization in COS7 cells.
目的克隆人血管紧张素转换酶2基因（ACE2），并构建其真核表达载体。
Objective To clone the human angiotensin-converting enzyme 2 (ACE2)and construct its eukaryotic expression plasmid.
HBV靶向核糖核酸酶真核表达载体的构建及其在2.2.15细胞内的表达。
Construction of HBV targeted ribonuclease and its expression in 2.2.15 cell line.
结论建立了两个高效表达突变CD59的真核表达系统，获得阳性克隆细胞株。
Conclusion a eukaryotic system that expressing mutant CD59 cDNA was successfully set up.
目的构建反义MBD1基因片段真核表达载体，为研究MBD1基因功能提供工具。
Objective to construct eukaryotic expression vector of antisense MBD1 gene fragment and to provide a tool for studying MBD1 gene function.
将构建好的兔防御素真核表达载体电击转化椭圆小球藻硝酸还原酶功能缺失突变体。
The eucaryotic NP-1 expression vector was transformed into the NR deficient mutant of c.
目的：构建反义vegf基因真核表达载体，研究其对肾癌细胞VEGF表达的影响。
AIM: to construct eukaryotic expression vector carrying human antisense VEGF gene and to study its effect on VEGF expression and growth of renal cell carcinoma.
真核表达的结果通过荧光染色的方法在流式细胞仪上进行检测，得到了明显的区分效果。
The consequence of eucaryon express proteinum binding with IBDV, then by fluorescence staining, detection on flow cytometer(FCM), we can gain a conspicuous discrimination result.
该腺病毒和真核表达质粒在肿瘤治疗药物、放射治疗增敏的制备中具有独特的实用价值。
The adenoviruses and the eukaryotic expression plasmid have unique practicality in preparing tumor treating medicines and radiotherapy hypersensitivity.
目的：构建人肝细胞生长因子（HGF）的真核表达载体，并在COS7细胞中进表达。
To construct PCI-hepatocyte growth factor (HGF) expression vector and to detect its transient expression in transfected COS7 cell line.
目的构建人G250真核表达载体，建立稳定表达人G250的小鼠黑色素瘤细胞系。
Objective To construct the human G250 eukaryotic expression vector and establish the stable B16 cell line expressing human G250 in mice.
构建小鼠il -4截短型基因真核表达质粒，表达小鼠il - 4受体拮抗体蛋白。
To clone mouse interleukin 4 (mIL-4) truncated gene, construct its eukaryotic expression plasmid pFB-mIL4 and express the truncated protein (murine IL-4 receptor antagonist).
实验结果表明：该系统使用合适的真核表达载体提供脊灰病毒结构蛋白的技术路线是可行的。
The result indicated: in this system, the technique of eukaryotic expression vector providing poliovirus capsid protein was possible.
目的克隆激活转录因子(atf)5，构建其真核表达载体，观察其在细胞中的表达定位。
Objective to clone the gene activating transcription factor 5 (ATF5), construct the expression plasmid and detect the localization of ATF5 in cultivated cells.
目的建立丙型肝炎病毒(HCV)复合多表位基因的真核表达载体，并在COS7细胞中瞬时表达。
Objective to construct an eukaryotic expression vector of compound multi-epitope gene of HCV and express the gene in COS7 cells.
目的：重组神经营养素3基因，在面神经损伤模型作肌肉真核表达，为治疗神经损伤打下科研基础。
Objective: To recombine the NT-3 gene and express NT-3 gene in the muscles of the model of facial nerve lesion and for providing an experimental basis in treating the injured peripheral nerve.
最后，对制备出的假病毒颗粒进行真核细胞感染实验，以间接免疫荧光法验证上述抗原的真核表达情况。
Immuno-fluorescence assay was used to confirm the expression of the recombinant proteins after virus infection in BHK21 cells.
目的克隆人促甲状腺激素受体胞外段基因，构建重组真核表达质粒，获得具有免疫学活性的纯化重组蛋白。
Objective to clone and construct the plasmid containing human thyroid stimulating hormone receptor (TSHR) gene ectodomain, and then identify the immunoreactivity of the purified recombinant protein.
构建突变人CD59分子（HMCD59）真核表达体系，探讨HMCD59糖基化前后抗补体活性的变化。
To construct human mutant CD59(HMCD59) eukaryot ic expression system and investigate whether glycation could inhibit the protection role of HMCD59 against human complement.
构建突变人CD59分子（HMCD59）真核表达体系，探讨HMCD59糖基化前后抗补体活性的变化。
To construct human mutant CD59(HMCD59) eukaryot ic expression system and investigate whether glycation could inhibit the protection role of HMCD59 against human complement.
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