建立完善的精原干细胞体外培养体系，为精原干细胞的体外大量扩增提供技术和方法，为治疗男性不育症等提供相关技术。
Finally, to establish the culture system of spermatogonial stem cells in vitro and to offer the therapy technique for the male sterility.
主要观察指标：隐睾的形态学变化，精原干细胞分选结果。
MAIN OUTCOME MEASURES: the morphological changes of cryptorchidism, and the sorting results of spermatogonial stem cells.
目的以原代培养小鼠骨髓基质细胞为饲养层，探讨精原干细胞能否在该饲养层上生长及生长的影响因素。
To study whether spermatogonial stem cells can grow on the feeder layers of bone marrow stromal cells, and their effecting factors in vitro.
本文就近年来胚胎干细胞和精原干细胞在男性生殖中的研究进展作一介绍。
This article reviews recent advances in studies of the use of embryonic stem cells and spermatogonial stem cells in male reproduction.
结果原代的小鼠培养骨髓基质细胞能很好的维持精原干细胞的非分化性扩增。
The feeder layers of bone marrow stromal cells allowed spermatogonial stem cells to proliferate well and remain undifferentiated.
应用细胞化学、免疫组织化学方法研究了体外培养小鼠精原干细胞的细胞化学和免疫组织化学特性。
Cytochemical and immunohistochemical characteristics of spermatogonial stem cell of culture in vitro were studied with cytochemistry and immunohistochemistry.
目的：建立体外研究精原干细胞与支持细胞共培养的细胞模型，探讨体外精原干细胞在支持细胞层上的增殖特点。
Objective: to construct cell model of spermatogonial stem cells (SSCs) with Sertoli cell layer in vitro, and study the proliferation characteristic of SSCs in vitro.
目的：建立精原干细胞的培养方法并对培养细胞的生物学特征进行研究。
Objective: To investigate the culture methods of rabbit spermatogonial stem cells (SSCs) and their biological characteristics in vitro.
精原干细胞的成功培养为今后重建完整的生精细胞系的治疗性移植和对这类定向干细胞的发育及分化潜能的研究提供了细胞模型。
The cultivation of SSCs may provide an ideal cell model for the research of committed stem cells' development and differentiation, which can be applied in remedial transplantation.
然而仍然不清楚miR - 34和哺乳动物精原干细胞(SSCs)之间的关系。
However, it is still unclear what the relationship is between miR-34c and mammalian spermatogonial stem cells (SSCs).
此方法借助于验证精原干细胞迁移的器官培养体系。
The method depends on an organ culture system onto which SSCs are transplanted.
因此，体外体系的建立也能用于修正或改进微环境条件使其满足鼠科动物精原干细胞系的生精发生需求。
Thus, an in vitro system is established that can be used to correct or manipulate the micro-environmental conditions required for proper spermatogenesis from murine SSC lines.
本文对精原干细胞的生物学特征，移植技术及其在医学领域的应用前景进行展望。
In this article, we show the the biology characteristic of SSCs, the transplantation technique and t...
我们的实验证实，在老鼠精原干细胞中，miR-34c通过与Nanos2+相互作用可能促 进减数分裂导致 Stra8 的上调。
Our experiments identified that miR-34c may promote meiosis process by interacting with Nanos2 leading up-regulation of Stra8 in mouse spermatogonial stem cells.
目的：探索及完善精原干细胞的分离、鉴定和体外培养技术；
Objectives:To establish the culture system of spermatogonial stem cells in vitro;
在老鼠中，我们研究发现miR - 34 c靶向作用Nanos2 +调节精原干细胞的分化。
We found that a conserved microRNA-34c through its target-Nanos2, regulating SSCs' differentiation in mouse.
在老鼠中，我们研究发现miR - 34 c靶向作用Nanos2 +调节精原干细胞的分化。
We found that a conserved microRNA-34c through its target-Nanos2, regulating SSCs' differentiation in mouse.
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