目的建立人感染高致病性禽流感病毒H5N1的核酸检测方法，用于人感染高致病性禽流感病毒疑似病例临床标本的检测。
Objective To establish a method for H5N1 RNA detection and laboratory diagnosis of suspected human avian influenza (H5N1) virus infected cases.
中国海关开始对所有入境点的入境人员进行核酸检测。
Chinese customs began NAT on inbound arrivals at all points of entry.
用脱氧核糖核酸末端转移酶介导的缺口末端标记技术（TUNEL）检测细胞凋亡。
Terminal deoxynucleotidyl transferase-mediated nick end labeling (TUNEL) technique were applied to deterimine the apoptotic cells.
血液制造商开始根据FDA的研究性新药(IND)申请程序执行核酸检测(NAAT)。
The Blood manufacturing community began implementation of Nucleic Acid Amplification Testing (NAAT) under the FDA's Investigational New Drug (IND) application process.
实时荧光定量PCR技术是一种利用荧光检测方法来定量核酸的技术，具有高度的灵敏性、特异性和精确性。
Real-time fluorescent quantitative PCR is a kind of technique that can quantify the nucleic acid on different fluorescence, as it features high sensitivity, accuracy, and specificity.
禽流感的检测大体可以分血清学检测和依赖于PCR技术的分子生物学检测，其中依赖核酸的扩增检测技术属于新型检测手段。
The methods of detecting AI include mainly serum-testing and molecular biologic methods depending on PCR, of which nucleic acid testing techniques depending PCR belongs to new detection methods.
荧光共振能量转移(FRET)技术作为一种高效的光学“分子尺”，在生物大分子相互作用、免疫分析、核酸检测等方面有广泛的应用。
Fluorescence resonance energy transfer (FRET) has broad applications in the study of the interactions of biological macromolecules, immunoassay, nucleic acid detection and so on.
本文回顾了各种新的纳米粒子与多核酸组成的系列用于DNA杂交电检测方法。
This article reviews a variety of new nanoparticle assembilies for advanced electrical detection of DNA sequences.
采用脱氧核糖核酸末端转移酶介导的缺口末端标记技术（TUNEL）和免疫组化方法检测鼻咽低分化鳞状细胞癌和正常鼻咽黏膜组织。
Terminal deoxynucleotidyl transferase-mediated nick end labeling (TUNEL) technique and immunohistochemistry method were applied to detect NPC tissue and normal nasopharyngeal tissue (NNPT).
结果表明，核酸探针是检测MDV灵敏特异的方法。
The results indicated that nucleic probe might be useful for the specific and sensitive assay of MDV.
聚合酶链反应(PCR)技术用于病毒核酸的检测具有灵敏度高，特异性强等优点，已广泛用于各型肝炎病毒的实验室检测。
Polymerase Chain Reaction (PCR) technique has been widely used in detecting different hepatitis viruses in laboratory, because of its high specificity and sensitivity.
目的探讨本地区性传播疾病（STD）患者沙眼衣原体（CT）、解脲支原体（UU）的感染现状及使用核酸扩增杂交梳法检测CT、UU的价值。
Objective To detect the status of CT and UU infection in local (STD)patients, and the value of measuring CT and UU with Nucleic Acid Amplification Hybridization Climb method.
本文综述了食品中病原微生物的快速检测方法研究进展，并对微生物培养法、免疫学方法和核酸分子杂交等现优检测方法进行了比较和评述。
The article introduces the development of rapid detections of pathogenic microbe in food, and reviews modern methods of microbiology, immunology, nucleic acid hybridization and so on.
方法对广西境内15种117只野生动物及99只人工养殖的果子狸采用聚合酶链反应和血清学方法检测其病毒核酸。
Methods Serological test and PCR method were used to detect 117 of 15 species of wild animals and 99 domesticated Paguma larvata.
温度梯度凝胶电泳(TGGE)是一种用于检测核酸序列变异和点突变的电泳方法。
Temperature gradient gel electrophoresis (TGGE) is a new and powerful electrophoresis method for separation of nucleic acids (DNA and RNA) and analysis of sequence variations.
这样的组装方法可以优化核酸识体在表面的固定，并且便于核酸识体在检测目标时的合拢。
This immobilization strategy optimizes the orientation of aptamer on the surface and facilitates the folding on binding target.
本研究建立了西方马脑炎病毒的RT-PCR检测方法，应用于实验感染西方马脑炎病毒的来亨鸡血液及蚊虫样本中的病毒核酸检测。
A pair of primers were synthesized, and related RT-PCR assay was established to detect the western equine encephalitis virus(WEEV)in experimentally infected mosquitoes and Leghorn chicken.
对临床标本的检测，也获得相似结果。结论：各试剂之间提取核酸的效率存在差异，应该选择性价比较好的方法进行临床标本的实验室RNA提取。
Conclusion: the results suggest that it had significant difference between four RNA extraction methods, so we should choose the appropriate one for detection of flu viruses in clinical specimens.
核酸杂交试验：是检测HPV感染的重要的手段，包括斑点印迹法（dotblothybridization）、组织原位杂交法、核酸印记法（Southern blot hybridization）。
Nucleic acid hybridization test: it is an important means to detect HPV infection, including dot blot method (blot hybridization dot), tissue in situ hybridization, blot hybridization (Southern).
用脱氧核糖核酸末端转移酶介导的缺口末端标记技术（TUNEL）原位检测细胞凋亡。
Terminal deoxynucleotidyl transferase-mediated nick end labeling (TUNEL) technique were applied to deterimine the apoptotic cells in all the tissues mentioned above.
目前人免疫缺陷病毒感染的实验室诊断主要依靠抗体检测，其他还包括P24抗原检测、核酸检测、病毒的分离培养等辅助检测手段。
Diagnosis of human immunodeficiency virus(HIV) infection mostly rely on the detection of HIV-1/2 antibody, P24 antigen, HIV RNA, virus culturing can be used as additional test.
结论利用肽核酸生物传感器成功地绕过了PCR扩增而直接检测出了临床标本中的HBV基因组dna。
CONCLUSIONS HBV DNA extracted from clinical samples were directly detected using PNA biosensor and PCR amplification was successfully bypassed.
结果：RT-PCR可在心脏、肝脏、脾脏、肾脏以及脑组织内检测到病毒核酸，透射电镜下可在肝细胞胞浆内观察到形态典型的病毒粒子。
R esu lts:The nuc le ic ac id of CCHF virus could be detected in the hearts, livers, spleens, kidneys and brains of the infected suckling mice by the RT-PCR.
而且核酸的检测可有助于对HIV早期感染的明确诊断。
Moreover, HIV viral load assay as a supplemental test would be useful for early diagnosis of HIV infection.
用核酸扩增荧光定量法检测血清、胃黏膜HBVDNA ，综合分析各检测值对肝胃不和证积分的意义。
Liver function and the markers of HBV were detected. The contents of HBV- DNA in serum and in gastric mucosa were assayed respectively by fluorescence quantitative polymerase chain reaction (FQ-PCR).
基于此，建立了核酸适配体识别的均相检测腺苷的新方法。
Based on it, a novel homogeneous aptamer recognizing based method for adenosine detection was established.
过去研究人员通过定位这种酶在基因组中的结合位点，之后检测这些序列是否受损的方法来寻找锌指核酸酶的错误。
In the past, researchers have looked for zinc-finger nuclease errors by assaying where in the genome the enzymes might bind, and checking to see whether those sequences were broken.
毛细管电泳(CE)是一种高分辨率、快速、敏感、能自动化的高效分离检测技术，可用于核酸的分离及检测。
Capillary electrophoresis (CE) is a rapid, sensitive, high-powered automated technique with higher resolution and has been used in detection of nucleotides.
毛细管电泳(CE)是一种高分辨率、快速、敏感、能自动化的高效分离检测技术，可用于核酸的分离及检测。
Capillary electrophoresis (CE) is a rapid, sensitive, high-powered automated technique with higher resolution and has been used in detection of nucleotides.
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