对复性后的单链抗体以抗原捕获ELISA法进行检测。
The refolded single-chain antibodies were examined by antigen capture ELISA.
为获取识别多种WSSV抗原的抗体提供了新的方法路线， 也为获取特异性识别线性表位的单链抗体提供了一种新的淘选技巧。
This study provides a new way to obtain more different antibodies specifically binding to WSSV, and especially provides a new strategy to obtain scFvs against linear epitopes.
目的探讨在不同的免疫学检测方法中，与单链抗体相连的亲和标记肽对单链抗体与抗原结合活性的影响。
Objective to investigate the affects of affinity tag on binding activity of single chain variable fragment (ScFv) to antigen using different immunological assays.
结论错配pcr结合噬菌体展示技术是提高单链抗体亲和力的一种有效且简便的手段。
Conclusion Error-prone PCR is an effective and simple method for affinity maturation of antibodies isolated from a phage antibody library.
为了降低人抗鼠抗体反应，获得满意的免疫原性，将模拟人卵巢癌抗原的抗独特型单链抗体人源化。
In order to reduce the human anti mouse antibody response and obtain optimal antigenicity, anti idiotype single chain which mimicking ovarian cancer antigen has been humanized.
从NCBI的数据库最近的同源性多重路线人士透露，单链抗体的获得的新序列。
Multiple alignments with the nearest homologies from the NCBI databases have revealed that the sequences of ScFv obtained are new.
目的观察抗PNAS - 2单链抗体与PNAS - 2抗原特异性结合的能力，并在此基础上探究其对急性单核细胞白血病u 937细胞凋亡的影响。
Objective to observe specific binding ability of anti-PNAS-2 single chain antibody with PNAS-2 antigen and its effects on apoptosis of human leukemic U937 cells.
拟获得在大肠杆菌中能高效可溶表达的转铁蛋白受体单链抗体与链亲和素（SA）的重组融合蛋白。
To obtain the anti-transferrin receptor scFv and streptavidin(SA) recombinant fusion proteins which were high-efficiently and solubly expressed in E.
本发明提供单链抗体定向的包含多官能连接基的聚合物前药。
The present invention provides single chain antibody-directed polymeric prodrugs containing multifunctional linkers.
溴脱氧尿苷脉冲标记细胞或组织后，BrdU （Bu20a）小鼠单克隆抗体可用于检测掺入单链DNA的BrdU。
After pulse-labeling cells or tissues with bromodeoxyuridine, BrdU (Bu20a) Mouse mAb can be used to detect BrdU incorporated into single stranded DNA.
溴脱氧尿苷脉冲标记细胞或组织后，BrdU （Bu20a）小鼠单克隆抗体可用于检测掺入单链DNA的BrdU。
After pulse-labeling cells or tissues with bromodeoxyuridine, BrdU (Bu20a) Mouse mAb can be used to detect BrdU incorporated into single stranded DNA.
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