因此，研究集中于改进病毒载体。
不同的病毒载体各有利弊。
Different viruses have their own advantages and disadvantages.
这项技术需要用到植入新基因病毒载体和多种调控蛋白。
The technique involves gene insertion by a virus vector and exposure to numerous regulatory proteins.
因此寻找新的非病毒载体成为目前的研究热点。
The recent topic has been focused on the new non-viral vectors.
这样注入细胞，他们就可以不必依赖于病毒载体。
文章综述了近年来牛腺病毒载体构建的一些研究进展。
The paper reviewed progress on the construction of bovine adenovirus vectors.
目的探索逆转录病毒载体在基因治疗乙型肝炎中的应用。
Objective To investigate the effectivness of recombined retrovirus vector in gene therapy.
重组病毒载体因其自然感染途径而成为有效的基因转移手段。
Recombinant viral vectors have become effective tools for gene transfer by means of their natural infective ways.
目的尝试利用腺病毒介导慢病毒载体的细胞感染以提高转染效率。
Purpose To test the hypothesis that adenovirus may improve gene transfer efficiency of lentiviral vector by mediating viral entry.
基因转移载体是基因治疗的重要组成部分，包括病毒载体和非病毒载体。
Gene transfer vector is the important ingredient in gene therapy, which includes virus vector and non-virus vector.
目的：比较鞘内注射第一代与第三代腺病毒载体后引起免疫反应的差异。
Objective:To examine the different immune responses between the first-generation and the third-generation Ad vectors by intrathecal injection.
他补充说：“较高的温度为病毒载体及病毒提供了增长生长繁殖速度的条件。
"Thesehigher temperatures provide for the vectors and the viruses to growfaster and produce more, " he added.
然而，最佳的导入入脑的病毒载体仍需探索，病毒载体的脑内分布也不确定。
However, optimal viral vector delivery into the brain is challenging and brain distribution of viral vectors is uncertain.
结果：逆转录病毒载体能有效地将外源性基因导入血管内皮细胞并稳定表达。
Results: The CGRP gene could be effectively transferred into HUVEC and stably expressed.
结果表明，自我失活型逆转录病毒载体能很好地用于衰老基因转录调控的研究。
The results demonstrate that self-inactivating retrovirus vector can be used for gene transcription regulation research during the aging process.
在利伯氏黑蒙的病例中，携带正常基因的病毒载体可以被直接注入视网膜而感染视网膜细胞。
In the case of Leber's congenital amaurosis, viruses carrying the correct gene can be injected directly into the retina where they will infect retinal cells.
目的：构建携带内皮抑素基因的增殖型腺病毒载体，寻找结肠癌的有效治疗方法。
Objective: To construct a replication-competent adenovirus vector carrying human endostatin gene, looking for an effective method for the therapy of colon cancer.
目前，NSC在恶性脑肿瘤中基因治疗的应用，主要包括构建载体和包装病毒载体。
At present, the applications of NSC gene therapy of malignant brain tumor mainly includes the construction of carriers and the package of viral vectors.
VSV的这些特点使其具有巨大的优势和潜力作为一种活病毒载体和肿瘤治疗载体。
The unique characteristics let the VSV have great potential and advantage to be a live vaccine vector and oncolysis viral vector.
结果合成的DNA转运系统和经修饰的病毒载体体外转染靶细胞后，可获得显著表达。
Results Artificial synthetic DNA transfer system and modified viral vectors could efficiently transfect target cells and get high level expression.
DNA缩合不仅是自然界常见的生理现象，也是非病毒载体介导基因转染的关键步骤。
DNA condensation is a common physiological phenomenon in nature and also a key step in non-viral vector-mediated gene transfection.
目的：探讨慢病毒载体在白血病细胞中的基因转导效率，为白血病基因治疗提供关键依据。
Objective to investigate the gene transduction efficiency of lentiviral vector in leukemia cells to provide key basis for leukemia gene therapy.
结论构建了含hpv16e7的逆转录病毒载体，为人关节软骨细胞的基因转染打下了基础。
Conclusion Successfully constructed retroviral vector encoding for HPV16E7 establishes important basis for gene transfection of human articular chondrocytes.
目的：构建及鉴定载入金属蛋白酶组织抑制因子1（TIMP鄄1）的重组腺相关病毒载体。
Objective: To construct and identify recombinant adeno-associated virus encoding rat tissue inhibitor of metalloproteinase-1(TIMP-1) full length cDNA.
该研究结束时，在细胞移植和为基因传输所进行的重组细胞病毒载体研发这两方面取得了进展。
To this end, advances have been made in the areas of cell transplantation and in the development of recombinant viral vectors for gene delivery.
目的构建携带双自杀基因且可诱导敲除SV40T的逆转录病毒载体，优化目前的肝细胞永生化。
Objective To construct a new retroviral vector with double-suicide genes and knockout-inducible SV40T gene, with the aim of optimizing immortalization of hepatocytes.
结论：杆状病毒载体能够介导HHV - 8肿瘤转化基因k12编码的蛋白在昆虫细胞中表达。
Conclusion: Baculovirus transfer vector may mediate the coding protein of HHV-8 K12 gene expressed in insect cells.
摘要腺病毒载体具有高效转染和低细胞毒性的特点，已成为基因治疗研究中应用最广泛的载体之一。
Adenovirus vectors have the characteristics of high transfection efficiency and low cytotoxicity, and they have become most widely used vectors for gene therapy.
摘要腺病毒载体具有高效转染和低细胞毒性的特点，已成为基因治疗研究中应用最广泛的载体之一。
Adenovirus vectors have the characteristics of high transfection efficiency and low cytotoxicity, and they have become most widely used vectors for gene therapy.
应用推荐