应用PCR方法检测组织细胞培养物中的病毒增殖和增殖滴度,测定了PCV2病毒收获时间,试验表明PCV2病毒在PK15组织培养可连续传代,增殖病毒。
PCR method was applied to detect the proliferation condition and titer of PCV2 and to determine the harvest time of PCV2. The results showed that PCV2 could proliferate and passage on PK15 cells.
应用PCR方法检测组织细胞培养物中的病毒增殖和增殖滴度,测定了PCV2病毒收获时间,试验表明PCV2病毒在PK15组织培养可连续传代,增殖病毒。
PCR method was applied to detect the proliferation condition and titer of PCV2 and to determine the harvest time of PCV2. The results showed that PCV2 could proliferate and passage on PK15 cells.
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