部分缺失的棉花曲叶病毒互补链基因启动子具有超强活性。
Partial Deleted Complementary Sense Gene Promoter from Cotton Leaf Curl Virus Shows Super Strong Promoter Activity.
在腺病毒载体的构建过程中，早期和晚期启动子的选择、构建以及去除末端蛋白(TP)、克隆末端序列是至关重要的。
The selection and cloning of early and late promoters and removal of adenovirus terminal protein (TP) are very important in construction of adenovirus vector.
目的：利用已有质粒构建带GF P报告基因的启动子鉴定质粒，并用此质粒鉴定丙型肝炎病毒5非翻译区(HCV 5'-UTR)启动基因表达的活性。
Objective; To construct a promoter identifying plasmid with GFP as reporter gene, and then identify the promoter activity of HCV 5 '- UTR with this construct.
结果表明：在双基因逆转录病毒载体介导的小鼠骨髓细胞的基因转导中，IRES与内部SV40启动子相比，更能保证双基因的共同表达。
Results showed that IRES was superior to SV40 promoter for guarantee of genes co expression in bicistronic retrovirus mediated gene therapy.
同时，我们还发现，病毒非结构蛋白NS1对该启动子具有反式激活作用，并且这种激活作用的大小取决于 NS1 蛋白在细胞中的浓度。
In addition, we found that the tr a nscriptional activity of this promoter could be transactivated by the viral nonstructural protein NS1 in a concentration dependent fashion in S2 cells.
结论EBV相关胃癌和EBV阳性鼻咽癌组织中病毒潜伏期基因启动子的甲基化状态不同，甲基化是调控EBV潜伏期基因启动子活动的重要机制之一。
Conclusion The methylation status of EBV latent gene promoters in EBVaGC and NPC is different, methylation is one of important mechanisms in regularing the activity of EBV latent gene promoters.
结论EBV相关胃癌和EBV阳性鼻咽癌组织中病毒潜伏期基因启动子的甲基化状态不同，甲基化是调控EBV潜伏期基因启动子活动的重要机制之一。
Conclusion The methylation status of EBV latent gene promoters in EBVaGC and NPC is different, methylation is one of important mechanisms in regularing the activity of EBV latent gene promoters.
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