目的探讨大鼠心脏移植过程中,重组腺病毒介导的外源基因转移至供心的可行性及安全性。
Objective to study efficiency and security of the recombinant adenoviral-mediated gene transfer to the donor heart during the heart transplantation.
逆转录病毒介导的基因转移技术要过渡到临床应用,主要解决如何使病毒载体具有高滴度而不具有复制活性。
In order to use retroviral-mediated gene transfer technology in clinical application, retroviral vector must be of high titer and free of detectable replication-competent retroviruses.
结论逆转录病毒介导的基因转移系统是高效、安全的,有助于人类白血病的基因治疗研究。
Conclusion the efficiency and safety of the gene transfer system may provide an optimal experimental system in gene therapy for human leukemia.
目的:观察腺病毒介导的基因转移在乳腺癌细胞的转导效率及腺病毒转导对细胞生长的影响。
Objective: To observe the transduction efficiency of adenoviral-mediated gene transfer in human breast cancer cells and the effect of viral transduction on the growth of the cancer cells.
目的构建人胰岛素样生长因子- 1的逆转录表达载体,建立逆病毒介导的IGF - 1基因转移系统。
Objective to construct a retroviral mediated expression system of human insulin-like growth factor-1 (IGF-1), and determine whether bone marrow stromal cells (BMSCs) can be infected by retrovirus.
目的构建人胰岛素样生长因子- 1的逆转录表达载体,建立逆病毒介导的IGF - 1基因转移系统。
Objective to construct a retroviral mediated expression system of human insulin-like growth factor-1 (IGF-1), and determine whether bone marrow stromal cells (BMSCs) can be infected by retrovirus.
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