目的探索可兼顾双向电泳凝胶图像质量和结果保真性的软骨蛋白提取方案。
Objective To explore an optimal cartilage protein extraction approach that can guarantee both the image quality and the result fidelity of the two-dimensional gel electrophoresis (2-DE) technique.
蛋白质印迹法:蛋白质分子从电泳凝胶转移到固相介质,然后用抗体进行免疫检测的技术。
Western blotting --- A technique analogous to Southern blotting, used for detection of proteins, usually by immunological methods.
在中间槽室中配置梳状板并采用琼脂糖凝胶封固电泳凝胶,可以从电泳凝胶中回收大分子样品组分。
A comb plate is arranged in the middle groove chamber, sepharose is used to seal the running gel, and macromolecule sample components can be recovered from the running gel.
经凝胶电泳分析,发现四对实验对象中有两对的酶的氨基酸序列略有差异。
Analysis by gel electrophoresis revealed slight differences in amino acid sequence of the enzymes of two of the four pairs.
分子量通过三辛凝胶电泳确定。
The molecular weight was determined by tricine gel electrophoresis.
橙色的塑料盒里装的是凝胶电泳,这是一种根据大小为dna测序的仪器。
And the orange plastic thing runs gel electrophoresis, a way to sort DNA strands by size.
因而他决定制造比市面价格便宜的实验基础工具凝胶电泳盒。
He decided to create a cheaper version of the gel-electrophoresis box, a basic tool used in a wide range of experiments.
在一个复杂的社会中是非常难侦测到一场大型爆发的疾病的源头的,但是脉冲场凝胶电泳的出现改变了一切。
In a complex society, it was difficult to detect widespread outbreaks, let alone trace those outbreaks to a specific source. PFGE changed everything.
用琼脂糖凝胶电泳检测两类卵的颗粒细胞凋亡。
Granulosa cell apoptosis was detected by agarose gel electrophoresis.
应用流式细胞术、DNA琼脂糖凝胶电泳、透射电镜等多种手段检测心肌细胞凋亡的改变。
The myocardial cells of each groups were taken for detecting apoptosis by the flow cytometry and DNA gel electro-phoresis.
结果:PCR产物经琼脂糖凝胶电泳分析,仅在包含绿脓杆菌基因组DNA的样品中得到单一条带。
Results:A single band was obtained on agarose gel electrophoresis only from PCR products that contained genomic DNA of P. aeruginosa.
目的:运用琼脂糖凝胶区带电泳进行蛋白尿的分析。
用琼脂糖凝胶电泳和细胞形态学观察细胞凋亡;
The thymocytes apoptosis was observed by agarose gel electrophoresis and cell morphology.
方法单细胞凝胶电泳。
需要使用包括聚合酶链反应(PCR),琼脂糖凝胶电泳,探针的标记与检测等生物工程技术。
This technique employs standard operations of biomedical engineering, such as polymerase chain reaction(PCR), agarose gel electrophoresis, labeling and detecting of probe.
结论改良琼脂糖凝胶电泳法测定血清ldh同工酶应用前景良好。
Conclusion It is good application prospects to determine the LDH isoenzyme by improvement agarose gel electrophoresis method.
用聚丙烯酰胺凝胶电泳鉴定其纯度。
The purity was identified by polyacrylamide gel electrophoresis.
用8%非变性聚丙烯酰胺凝胶电泳将酶切产物分离,并用银染法显色。
The products of endonuclease digestion were run on 8% non-denaturing polyacrylamide gel electrophoresis and detected by silver staining.
聚丙烯酰胺梯度凝胶电泳显示一条GGT活性区带。
Gradient gel electrophoresis showed only one band of GGT activity.
目的初步探索碱性单细胞凝胶电泳(SCGE)方法用于电离辐射生物剂量估算的可行性。
Objective To explore the feasibility of alkaline single cell gel electrophoresis (SCGE) in biological dosimetry of ionizing radiation.
横向交变凝胶电泳。
所得纯化物以1%琼脂糖凝胶电泳鉴定超螺旋质粒的含量。
The level of purified superhelix plasmids were measured by 1% agarose gel electrophoresis.
单细胞凝胶电泳(彗星实验)检测细胞DNA的损伤。
DNA damage of germ cells was assessed by single cell gel electrophoresis (SCGE or Comet assay).
方法:可溶性蛋白质凝胶电泳。
用中性单细胞凝胶电泳方法检测辐射后DNA双链断裂(尾矩)。
Detecting DNA double-strand breaks(tail moment, TM) after radiation with neutral single cell gel electrophoresis.
用荧光染色法和琼脂糖凝胶电泳观察细胞凋亡情况。
Fluorescence staining and agarose gel electrophoresis were used to study apoptosis.
方法建立随机扩增多态性DNA聚丙酰胺凝胶电泳(RAPD-PAGE)指纹分型法;
Methods To establish a fingerprinting method by random amplified polymorphic DNA and polyacrylamide gel electrophoresis (RAPD-PAGE).
细胞形态学变化和DNA凝胶电泳观察细胞凋亡现象。
Cell morphology and DNA gel electrophoresis were used to observe the apoptosis.
目的研究不同厚度的琼脂糖凝胶对电泳的影响。
Objective to study different thick agarose gel how to impact the DNA electrophoresis.
目的研究不同厚度的琼脂糖凝胶对电泳的影响。
Objective to study different thick agarose gel how to impact the DNA electrophoresis.
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