用8%非变性聚丙烯酰胺凝胶电泳将酶切产物分离，并用银染法显色。
The products of endonuclease digestion were run on 8% non-denaturing polyacrylamide gel electrophoresis and detected by silver staining.
用酶切和PCR的方法对重组质粒进行鉴定。
Used restrict enzyme and PCR to verify the reconstructed plasmid.
目的建立尿中性肽内切酶(NEP)检测的ELISA法，明确用ELISA法检测尿nep在诊断肾小管损伤中的意义。
Objective to set up an ELISA method to measure urinary neutral endopeptidase (NEP) and determine its clinical meaning in diagnosing renal tubular injury.
用9个玉米和水稻线粒体基因探针与酶切条带杂交发现，不育系与保持系线粒体DNA的相同大小酶切片段含有相似的顺序。
Homologous sequences of mtDNA were found between MS and maintainer lines by using 9 probes of maize and rice to hybridize with digested mtDNA.
方法：用甲基化敏感的限制性核酸内切酶消化，结合聚合酶链反应（PCR）技术。
Methods: 20 cases of MDS patients were studied using methylation sensitive restriction enzyme digestion and polymerase chain reaction(PCR) technique.
乐复能以胰蛋白酶酶切后，用HPLC分析肽图；
The peptide map of novaferon was obtained by trypsin digestion and HPLC assay.
阳性重组质粒经pcr、酶切鉴定，用双脱氧链末端终止法进行序列测定。
The positive recombinant plasmid was identified by PCR, enzyme digestion. The nucleotide sequence of CSP3 'ending gene was determined by the dideoxy chain termination method.
方法对116例随机收集的1型糖尿病患者用聚合酶链反应-限制性内切酶消化作该点突变的筛选；
Methods The mutation had been screened from 116 unrelated patients with type 1 diabetes mellitus by ApaI digestion of product of polymerase chain reaction amplification.
方法对116例随机收集的1型糖尿病患者用聚合酶链反应-限制性内切酶消化作该点突变的筛选；
Methods The mutation had been screened from 116 unrelated patients with type 1 diabetes mellitus by ApaI digestion of product of polymerase chain reaction amplification.
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