目的：建立体外培养兔角膜缘上皮细胞的方法，观察其体外生长的生物学特性。
Objective:To establish a method for culturing limbal corneal epithelial cells of rabbit and to observe the growth characteristics of the cultured cells in vitro.
目的：探讨各种生长因子及其受体在正常角膜和角膜缘的表达及分布规律。
Purpose: To investigate the expression and distribution characteristics of growth factors and their receptors in normal cornea and limbus .
方法应用消化培养法对人胎儿角膜缘上皮组织进行体外培养并观察记录培养细胞的生长特性；
Methods Human fetal limbal epithelial tissue was cultured in vitro by enzymatic digestion culture method and the morphological characteristics of cultured cells were recorded.
结果10例新鲜角膜缘上皮组织培养后，7例有上皮细胞生长，1周形成细胞单层；
Results Cells growth occ urred in7of10pieces of fresh limbal tissue and formed monolayer cells in1wee k.
叶沿茎松散生长或成半莲座型，叶倒披针形，骤尖，3 -6厘米长1 -2，4厘米宽，6-8毫米厚，绿色，叶缘红。
Leaves diffuse along the stem or subrosulate, oblanceolate, cuspidate, 3 – 6 x 1 – 2,4 cm, 6 - 8 mm thick, green, margin red.
结果：倒置显微镜观察，悬浮细胞培养法A组和组织块培养法B组扩增的角膜缘上皮细胞均能在羊膜上单层生长。
Results: A monolayer of cultured corneal limbal epithelial cells was observed by inverted microscope in both A group and B group.
在对照组中，6只左眼结膜生长至角膜缘，右眼中2只结膜生长至角膜缘，4只形成结膜囊肿，两者之间有显著性差异。
In Cons, 6 left eyes were extended to limbal, 2 right eyes were extended to limbal, 4 right eyes formed conjunctiva cyst . There were significant difference between of them.
不同生长季节不同林型的根际蔗糖酶活性均高于林缘，差异均显著。
Enzymatic activity of invertase of root is higher than it on the forest edge, the differences are obvious.
以枸骨和全缘冬青作为嫁接砧木，均能取得较好的成活率，但采用枸骨嫁接其成活率和新梢的生长更好；
The survival ratio was preferable with the stocks of Ilex cornuta and Ilex Integra, between which the Ilex cornuta showed better.
以枸骨和全缘冬青作为嫁接砧木，均能取得较好的成活率，但采用枸骨嫁接其成活率和新梢的生长更好；
The survival ratio was preferable with the stocks of Ilex cornuta and Ilex Integra, between which the Ilex cornuta showed better.
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