遗传物质的多态性是基因功能调节的重要方式之一,基因编码区域单个核苷酸碱基的替换就可能改变蛋白质的结构和功能,对细胞或机体的生理功能产生重要影响。
Genetic Polymorphism is a important regulating type for the gene function, it can influence the protein structure and function by only change a single nucleotide in the coding region.
方法对来自中国不同地域的松杨栅锈菌的5个生理小种11个菌系核糖体its序列和微卫星序列多态性进行了研究。
ITS sequences and ISSR markers based on 11 isolates of 5 RACES collected from different parts in China were studied.
应用23个随机引物对白粉病菌8个不同生理小种进行RAPD分析,其中11个引物的扩增带型在不同生理小种间出现差异,共扩增出59条谱带,多态性为47.5%。
Differences were found among 8 strains from RAPD analysis. Out of 23 random primers, 11 were polymorphic. Altogether 59 RAPD bands were found and the polymorphic rate was 47.5%.
应用23个随机引物对白粉病菌8个不同生理小种进行RAPD分析,其中11个引物的扩增带型在不同生理小种间出现差异,共扩增出59条谱带,多态性为47.5%。
Differences were found among 8 strains from RAPD analysis. Out of 23 random primers, 11 were polymorphic. Altogether 59 RAPD bands were found and the polymorphic rate was 47.5%.
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