本文研究酶促和光促两法进行生物素标记DNA。
In this paper, enzymatic and ph? Otometic methods for labeling DNA with biotin were studied.
根据生物素标记反应的不可逆性,设计了一种基于磁性分离的快速纯化方法。
According to the irreversibility of biotin labeling, a quick method was designed for the purification of biotin labeling small molecule by magnetic separation.
经生物素标记探针杂交确认RANTES基因的整合,用免疫斑点印迹法对重组基因表达水平进行定量测定。
The integration of RANTES gene was confirmed by hybridization with biotin-labelled probes and the recombinant gene expression level was quantitatively detected by immunodot blotting.
为了测定注射后生物材料的结果,小鼠前壁心肌梗塞7天后钙离子交联型生物素标记海藻酸钠被注入梗塞部位。
To determine the outcome of the biomaterial after injection, calcium-crosslinked biotin-labeled alginate was injected into the infarct 7 days after anterior myocardial infarction in rat.
应用生物素标记HBVDNA(乙肝病毒脱氧核糖核酸)作探针,对129例肝病患者肝组织作原位杂交研究。
A biotin-labelled DNA specific for hepatitis B virus (HBV) nucleo-tide sequences was hybridized in situ to liver tissue of 129 cases with liver diseases.
采用链霉亲和素包被磁纳米粒子,将生物素标记的特异性抗体偶联在磁纳米粒子上,制备出高特异性的磁纳米探针;
The magnetic nanoparticle probe was prepared by specifically connecting the streptavidin-conjugated magnetic nanoparticles and the antibody of analyte via the strong streptavidin-biotin interaction.
PNA分子可被生物素和各种荧光团常规标记。
The PNA molecules can routinely be labeled with biotin or various fluorophores.
本产品已经过优化,可应用于检测生物素化的蛋白标记。
This product has been optimized to detect biotinylated protein markers.
本实验对生物素化补骨脂素标记核酸的条件和效果进行了研究,发现该试剂不仅能标记双链dna、单链dna,更重要的是可以直接标记RNA及寡核苷酸。
We have systematically studied the relations between the probe labelling effects and conditions, and found that it not only can label dsDNA, ssDNA, but also RNA and oligonucleotide.
采用本室建立的标记抗生物素-生物素酶联免疫试验及间接酶免疫试验进行血清与肝组织前S蛋白检测。
Labelled avidin-biotin-ELISA (LAB-ELISA) was established to detect pre-S proteins in serum, and it is verified that specificity of this technique is fairly high as compared with control experiments.
另一条为检测探针,3端标记生物素。
我们提供反应荧光探针和发光探针,生物素和端粒酶能够应用于标记药物小分子和生物聚合物,如蛋白、核酸以及其他碳水化合物;
Our reactive fluorescent and luminescent probes, biotins and tag enzymes are used for labeling small drug molecules and biopolymers, e. g, proteins, nucleic acids and carbohydrates;
我们提供反应荧光探针和发光探针,生物素和端粒酶能够应用于标记药物小分子和生物聚合物,如蛋白、核酸以及其他碳水化合物;
Our reactive fluorescent and luminescent probes, biotins and tag enzymes are used for labeling small drug molecules and biopolymers, e. g, proteins, nucleic acids and carbohydrates;
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