碱裂解法抽提质粒,琼脂糖电泳分析目的质粒。
The plasmid was obtained and identified by alkali splitting and gel electrophoresis.
用琼脂糖电泳法和乳胶凝集法(LAT)进行检测和比较。
Gel electrophoresis and latex agglutination test (lat) were employed to detect virus.
琼脂糖电泳纯化的HDL方法简便易行,适用于研究脂蛋白生理功能。
The purification method of HDL was easily carried out and suitable for study the function of lipoproteins.
亲本间分子量差异大的SSR位点可用高浓度琼脂糖电泳进行快速鉴定。
The SSR loci with great fragment length different between the parents should be selected in order to identify the genotypes by agarose gel.
经纸层析,醋酸纤维素薄膜电泳及琼脂糖电泳分析表明,其主成分为单一斑点。
It showed a single spot when identified by means of the paper chromatography, cellulose acetate electrophoresis and agarose electrophoresis.
三种多糖经HPLC、琼脂糖电泳和紫外光谱等方法检测,证明均为不含蛋白、核酸的均一组分。
Three. fractions were shown to be homogeneous by cellulose acetate membrane electrophoresis, agarose electrophoresis and HPLC.
本文用琼脂糖电泳观察国产三尖杉酯碱(H)及高三尖杉酯碱(H.H)与白血病细胞DNA的作用模式。
The pattern of China-made H and H. H act on leukemia cellular DNA was observed by the agarose electrophoresis analysis.
结果可以观察到清楚明亮的DNA条带,使大、中学师生同时掌握了正规dna提取和琼脂糖电泳两项技能。
Results To observe the effect of clear and bright electrophoresis strip, we made the student mastery of this technical ability of regular rapid pick-up and gelose electrophoresis appraisal of DNA.
所得极低密度脂蛋白(VLDL)及低密度脂蛋白(LDL)经琼脂糖电泳、聚丙烯酰胺电泳(PAGE)及载脂蛋白SDS-聚丙烯酰胺电泳(SDS-PAGE)鉴定纯度良好。
The purity of isolated VLDL and LDL was confirmed by the lipoprotein electrophoresis on agarose gel and PAGE and by the apolipoprotein electrophoresis on SDS-PAGE.
用琼脂糖凝胶电泳检测两类卵的颗粒细胞凋亡。
Granulosa cell apoptosis was detected by agarose gel electrophoresis.
目的:运用琼脂糖凝胶区带电泳进行蛋白尿的分析。
结果:PCR产物经琼脂糖凝胶电泳分析,仅在包含绿脓杆菌基因组DNA的样品中得到单一条带。
Results:A single band was obtained on agarose gel electrophoresis only from PCR products that contained genomic DNA of P. aeruginosa.
用琼脂糖凝胶电泳和细胞形态学观察细胞凋亡;
The thymocytes apoptosis was observed by agarose gel electrophoresis and cell morphology.
需要使用包括聚合酶链反应(PCR),琼脂糖凝胶电泳,探针的标记与检测等生物工程技术。
This technique employs standard operations of biomedical engineering, such as polymerase chain reaction(PCR), agarose gel electrophoresis, labeling and detecting of probe.
采用血清琼脂糖凝胶区带电泳技术分析未经处理和处理后的蛋白尿样本。
The serum agarose gel zone electrophoresis technique was adopted to analyze the non-treated and treated samples of proteinuria.
结论改良琼脂糖凝胶电泳法测定血清ldh同工酶应用前景良好。
Conclusion It is good application prospects to determine the LDH isoenzyme by improvement agarose gel electrophoresis method.
所得纯化物以1%琼脂糖凝胶电泳鉴定超螺旋质粒的含量。
The level of purified superhelix plasmids were measured by 1% agarose gel electrophoresis.
用荧光染色法和琼脂糖凝胶电泳观察细胞凋亡情况。
Fluorescence staining and agarose gel electrophoresis were used to study apoptosis.
方法应用PCR方法和琼脂糖凝胶电泳对92例不明原因共济失调先证患者测定其SCA1、SCA2和SCA3基因片段长度。
Methods The length of genetic segment of SCA1, SCA2 and SCA3 in 92 patients with ataxia of unknown origin were detected by polymerase chain reaction (PCR) and agarose gel electrophoresis.
方法:通过DNA结合溴乙锭后的荧光损失和DNA琼脂糖凝胶电泳的迁移率来测定dna的损伤程度。
Methods: DNA damage was measured by the loss of fluorescence after DNA binding to ethidium bromide and by the increased DNA mobility in agarose gel electrophoresis.
目的研究不同厚度的琼脂糖凝胶对电泳的影响。
Objective to study different thick agarose gel how to impact the DNA electrophoresis.
结果经琼脂糖凝胶电泳和DNA序列测定证实,建立的PCR检测方法具有极高的灵敏度和较好的特异性。
The results of agarose gel electrophoresis and DNA sequence analysis indicated that the PCR method had high specificity and sensitivity.
含2 %PVP的1 %琼脂糖凝胶电泳纯化,用DNA凝胶回收试剂盒回收后没有得到纯化后的土壤微生物总DNA。
Soil microbial total DNA could not be obtained through DNA gel extraction kit after purification with 1% Sepharose containing 2% of PVP (polyvinylpyrrolidone) agarose gel electrophoresis.
应用流式细胞术、DNA琼脂糖凝胶电泳、透射电镜等多种手段检测心肌细胞凋亡的改变。
The myocardial cells of each groups were taken for detecting apoptosis by the flow cytometry and DNA gel electro-phoresis.
HE染色、原位末端标记及琼脂糖凝胶电泳等实验证明,细胞的死亡方式为调亡。
The tests such as HE dye, still end labeling and gelose gel electrophoresis etc. have proved that the manner of cell death is apoptosis.
主要观测指标:紫外分光光度计检测吸光率(A值)及纯度、琼脂糖凝胶电泳、目的基因测序。
MAIN OUTCOME MEASURES:Ultraviolet spectrophotometer absorbance (A) and purity, agaroses gel electrophoresis, and objective gene sequencing.
经琼脂糖凝胶电泳分析载体与DNA结合力,并通过紫外分光光度计检测其载药量和包埋率;
The potential of adsorbing DNA on nanoparticles was analyzed by agarose gel electrophoresis and spectrophotometer.
用于电泳介质的琼脂糖最佳浓度为2%。
As media of electrophoresis, optimum concentration of agarose was 2%.
目的制定本实验室琼脂糖凝胶电泳法(age)测定血清蛋白的正常参考值范围。
Objective to establish the normal reference values of serum protein with agarose gel electrophoresis (AGE) in local clinical laboratory.
目的制定本实验室琼脂糖凝胶电泳法(age)测定血清蛋白的正常参考值范围。
Objective to establish the normal reference values of serum protein with agarose gel electrophoresis (AGE) in local clinical laboratory.
应用推荐