探索具有可放大的、非特异性纯化目的抗体片段的工艺路线。
To explore the antibody fragment purification technics that is befitting for production scale.
酶免疫分析技术的质量依赖于抗原的纯度、抗体的特异性、合适标记酶的选用，其灵敏度取决于标记酶的高度纯化和高转化率。
The quality of an enzyme immunoassays depends very much on the purity of the antigen or hapten used for conjugation, the specificity of the antibody and the choice of a suitable enzyme label.
方法用RT - PCR方法从能分泌特异性抗人afp单克隆抗体的杂交瘤细胞中分离纯化抗体VH和VL基因。
Methods VH and VL genes of anti-human AFP monoclonal antibody were cloned by RT-PCR from hybridoma.
结果：该方法纯化所得的自身抗体，能特异性地结合其所针对的靶抗原。
Results:The BMZ-Ab purified by this method could specific ally combine its target-antigen.
实验表明纯化后的抗体具有较高特异性。
Western Blot assay shows the purified antibody have higher specificity.
目的构建表达P30蛋白抗原表位的重组质粒及工程菌，获得纯化的P30蛋白抗原表位，用于检测弓形虫特异性抗体。
Objective To construct a recombinant plasmid containing P30 gene of Toxoplasma gondii and obtain recombinant protein Methods P30 gene fragment was amplified from genomic DNA of T.
目的构建表达P30蛋白抗原表位的重组质粒及工程菌，获得纯化的P30蛋白抗原表位，用于检测弓形虫特异性抗体。
Objective To construct a recombinant plasmid containing P30 gene of Toxoplasma gondii and obtain recombinant protein Methods P30 gene fragment was amplified from genomic DNA of T.
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