探讨大鼠牙囊细胞体外培养的方法。
To explore the culture method of rat dental follicle cells in vitro.
牙囊是牙齿和牙龈之间的空隙。
改良组织块法是一种良好的大鼠牙囊细胞培养方法。
Improved explant culture was a good culturing method for rat DF cells.
什么是牙囊?
发育中的骨小梁侵入牙囊接触到了牙胚导致釉质发育的障碍。
The developing bone trabeculae invaded the dental follicle and reached the molar tooth germs, provoking deformities in enamel surfaces. No root formation was observed.
本文根据颌骨含牙囊肿X线影像上的差异,将其分成四种类型。
According to the X-ray features of the dentigerous cysts in jaws, four radiologic types of these cysts were presented.
阻生尖牙邻近切牙的根吸收组和无根吸收组尖牙的牙囊大小有无差别。
The extent and location with the root resorption of neighboring incisors were observed in the sequential transaxial views;
结论:利用多次差速传代可从混合培养的原代细胞中获得纯化的牙囊细胞。
Conclusion: Dental follicle cells can be purified by several differential passages from the mixed primarily cultured cells.
方法:总结46例含牙囊肿病例,分析囊肿对邻近器官及解剖结构的影响。
Methods:46 cases of dentigerous cysts were selected. We analyzed the position of these cysts, their adjacent normal structures and normal erupted teeth.
结论:培养的人牙囊细胞具有合成与分泌egf的能力,流体静压力可以影响体外培养的人牙囊细胞egf的分泌。
Conclusion: the results indicated that EGF was synthesized and secreted by the dental follicle cells, and the amount of EGF secreted was influenced by different hydrostatic pressures.
结论:合适浓度的IGF-1能促进牙囊细胞的增殖,增加蛋白含量及向成骨细胞(成牙骨质细胞)方向分化的能力。
Conclusion:Proper concentrations of IGF-1 may promote dental follicle cell proliferation, protein synthesis and differentiation towards osteoblasts(cementoblasts).
骨桥蛋白和骨钙素的表达也呈阳性,与活体组织原位杂交的实验结果相符合,表明牙囊细胞具有合成矿化组织的能力。
The results indicated that dental follicle cells not only exhibit fibroblastic characteristics, but also have some characteristics of osteoblasts or cementoblasts.
骨桥蛋白和骨钙素的表达也呈阳性,与活体组织原位杂交的实验结果相符合,表明牙囊细胞具有合成矿化组织的能力。
The results indicated that dental follicle cells not only exhibit fibroblastic characteristics, but also have some characteristics of osteoblasts or cementoblasts.
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