研究人员分析了突变蛋白的功能,发现它是该蛋白激发激酶作用的部分,是酶缺矢功能的核心环节。
The researchers analyzed the function of the mutant proteins, establishing that it was the "triggering" kinase segment of the protein that was central to the enzyme's defective function.
目的:观察体外藤黄菌素对重组人蛋白激酶CK2的抑制效果及进行酶动力学分析以确定其抑制作用类型。
AIM: to observe the inhibitory effect of luteolin on recombinant human protein kinase CK2 holoenzyme and to confirm the inhibitory type by its enzyme kinetic analysis in vitro.
目的分析细胞周期素依赖激酶4 (CDK4)在颌骨软骨肉瘤的表达及意义。
AIM To analyze the expression and significance of cyclin dependent kinase 4 (CDK4) in chondrosarcoma of the jaws.
实验采用核蛋白激酶基因内含子5(AK5)和DNA测序法来分析这18种鸟的分子系统发育关系。
In this experiment, phylogenetic relationships of 18 species of passerines were studied using Adenylate Kinase Intron 5 (AK5) sequences and DNA techniques.
为了寻找肿瘤细胞内ck2的特异性抑制剂,本研究观察黄芩甙体外对重组人蛋白激酶CK2的抑制效果,并进行酶动力学分析以确定其抑制作用类型。
This study was to search specific CK2 inhibitors in tumor cells through observation of the inhibitory effects of baicalein on recombinant human protein kinase CK2 holoenzyme and its kinetics in vitro.
为了寻找肿瘤细胞内ck2的特异性抑制剂,本研究观察黄芩甙体外对重组人蛋白激酶CK2的抑制效果,并进行酶动力学分析以确定其抑制作用类型。
This study was to search specific CK2 inhibitors in tumor cells through observation of the inhibitory effects of baicalein on recombinant human protein kinase CK2 holoenzyme and its kinetic.
目的分析和探讨高血压脑出血用尿激酶溶凝引流治疗的效果。
Objective To discuss the effects of Burr hole intubation urokinase drainage for treating hypertensive cerebral hemorrhage.
SDSPAGE分析表明,鸡肌腺苷酸激酶的含量可占大肠杆菌细胞总蛋白含量的38%。
SDS PAGE analysis demonstrated that the enzyme was produced to the extent of as much as 38% of the total cellular protein.
激酶缓冲液用来分析蛋白激酶的活性。
目的建立一种灵敏、特异检测血清中二磷酸核苷激酶(NDPK -A)含量的酶联免疫吸附试验(elisa)法和试剂盒,分析血清中的NDPK - A含量与血液病之间的关系。
Objective to establish a sensitive and a specific ELISA kit for detecting and analysing serum NDPK-A and to explore the relationship between serum NDPK-A content and hemopathy.
采用文献资料分析法,综述了近40年来国内外对肌酸激酶研究的成果和运动训练对血清肌酸激酶活力变化影响的各种因素。
The present study summarizes achievements of research of creatine kinase(CK) and factors of sports training influencing serum CK activity in the past 40 years by literature.
经过分类分析,下调基因,主要可以分为五个功能类:蛋白质降解、RNA转录调控、细胞壁合成降解、激酶受体和肽运输。
The down-regulated genes can be divided in five classes:protein degradation, RNA transcription, cell wall synthesis and degradation, kinase receptor and peptide transport.
序列分析表明,其结构与所有已知的抗真菌蛋白不同,而与富含半胱氨酸的植物类受体激酶的胞外结构域相似。
The protein showed no similarity to other pathogenesis-related proteins but did show homology to the extracellular domain of plant cysteine-rich receptor-like kinases.
方法:对原发性高血压伴胰岛素抵抗患者胰岛素受体基因酪氨酸激酶域外显子17、18基因突变进行检测,并对突变的外显子进行核苷酸序列分析。
Methods: Using PCR SSCP, we detected the mutation of INSR tyrosine kinase domain exon 17 and exon 18 in 33 primary hypertension patients and 28 normal persons.
采用氨基酸自动分析仪测定了蚓激酶副产物中氨基酸的含量和组成。
The contents and compositions of amino acids of the by product in producing earthworm kinase (BEK) was determined by automatic analytical instrument of amino acids.
方法采用全自动生化分析仪检测血清肌酸激酶(CK)、肌酸激酶脑型同工酶(CK-BB)、乳酸脱氢酶(LDH)以及天门冬氨酸氨基转换酶(AST)的含量。
Methods Serum cytosolic enzyme creatine kinase (CK) and its brain band isoenzyme (CK BB), lactate dehydrogenase (LDH) and aspartate aminotransferase (AST) were measured with autobiochemical analyzer.
血清样品分析表明,添加CMH使血清中肌酸激酶活性提高了18.45%(P<0 .01),而对血清中肌醉、尿素氮等其它指标影响不显著。
The serum sample analysis indicated that CMH increased the activity of CK by 18.45%(P<0.01), but had no effect on the serum creatinine and other data.
应用受体的放射配基结合分析法测定出甜菜碱对大鼠肝细胞膜表皮生长因子受体(EGF)及其酪氨酸蛋白激酶(TPK)有显著的结合抑制作用。
Using radioligand of binding assay of receptors determinates that betaine can inhibit the binding of EGF receptor with EGF and affect the TPK activity conspicuously.
应用受体的放射配基结合分析法测定出甜菜碱对大鼠肝细胞膜表皮生长因子受体(EGF)及其酪氨酸蛋白激酶(TPK)有显著的结合抑制作用。
Using radioligand of binding assay of receptors determinates that betaine can inhibit the binding of EGF receptor with EGF and affect the TPK activity conspicuously.
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