将反义蜡质基因和潮霉素抗性基因通过共转化方法导入了籼稻9311，并进行了初步检测。
The antisense Waxy gene and hygromycin resistant gene were co-transformed into Indica rice 9311 and passed the basic detection.
T1和T2代中潮霉素抗性表现为显性单基因位点的遗传方式，符合孟德尔分离规律，并得到分子鉴定结果的证实。
Mendelian inheritance of hygromycin resistance was showed as single dominant locus and proved by Southern blotting analysis in T1 and T2 generation of all transgenic plants.
潮霉素抗性浓度的筛选为检测谷子转几丁质酶基因奠定基础。
The resistance concentration of hygromycin selected is the basis for testing transgenes millet plant.
对部分经潮霉素筛选得到的再生植株进行了多次重复P CR检测，发现其中40 %以上的潮霉素抗性植株均表现出较强的阳性反应，初步证明几丁质酶基因已整合到油菜细胞核基因组中。
PCR test of the resistant plants indicated that 40% of the Hyg-resistant plants showed strong positive reaction, suggesting that chitinase gene had been integrated into the genome of rapeseed.
对部分经潮霉素筛选得到的再生植株进行了多次重复P CR检测，发现其中40 %以上的潮霉素抗性植株均表现出较强的阳性反应，初步证明几丁质酶基因已整合到油菜细胞核基因组中。
PCR test of the resistant plants indicated that 40% of the Hyg-resistant plants showed strong positive reaction, suggesting that chitinase gene had been integrated into the genome of rapeseed.
应用推荐