潮霉素抗性浓度的筛选为检测谷子转几丁质酶基因奠定基础。
The resistance concentration of hygromycin selected is the basis for testing transgenes millet plant.
幼胚诱导的愈伤组织，经潮霉素抗性筛选，得到可育的再生植株。
The regenerated fertile plants were obtained by effective selection for hygromycin B resistance.
将反义蜡质基因和潮霉素抗性基因通过共转化方法导入了籼稻9311，并进行了初步检测。
The antisense Waxy gene and hygromycin resistant gene were co-transformed into Indica rice 9311 and passed the basic detection.
T1和T2代中潮霉素抗性表现为显性单基因位点的遗传方式，符合孟德尔分离规律，并得到分子鉴定结果的证实。
Mendelian inheritance of hygromycin resistance was showed as single dominant locus and proved by Southern blotting analysis in T1 and T2 generation of all transgenic plants.
对部分经潮霉素筛选得到的再生植株进行了多次重复P CR检测，发现其中40 %以上的潮霉素抗性植株均表现出较强的阳性反应，初步证明几丁质酶基因已整合到油菜细胞核基因组中。
PCR test of the resistant plants indicated that 40% of the Hyg-resistant plants showed strong positive reaction, suggesting that chitinase gene had been integrated into the genome of rapeseed.
对部分经潮霉素筛选得到的再生植株进行了多次重复P CR检测，发现其中40 %以上的潮霉素抗性植株均表现出较强的阳性反应，初步证明几丁质酶基因已整合到油菜细胞核基因组中。
PCR test of the resistant plants indicated that 40% of the Hyg-resistant plants showed strong positive reaction, suggesting that chitinase gene had been integrated into the genome of rapeseed.
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