方法:采用新鲜猪血抗凝离心后取血球,水浴加热溶血后,用3%盐酸丙酮提取血红素,提取液加鞣酸/氯化锶沉淀分离出血红素。
Methods:Red blood cell(RBC) was isolated from the anti-coagulated fresh pig blood, It was hemolyzed by heated water, then heme was extracted with 3% acetone-hydrochloric acid.
方法:采用新鲜猪血抗凝离心后取血球,水浴加热溶血后,用3%盐酸丙酮提取血红素,提取液加鞣酸/氯化锶沉淀分离出血红素。
Methods:Red blood cell(RBC) was isolated from the anti-coagulated fresh pig blood, It was hemolyzed by heated water, then heme was extracted with 3% acetone-hydrochloric acid.
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