目的:观察5 -溴脱氧尿嘧啶核苷标记肌源性干细胞的效果,探讨其最佳标记浓度和时间。
OBJECTIVE: to observe the effect of 5-bromodeoxyuridine (Brdu) labeling of muscle-derived stem cells in vitro, to study the optimal dosage and timing.
悬浮培养及贴壁培养的人和鼠神经干细胞,经抗巢蛋白及抗5 -溴脱氧尿嘧啶核苷免疫细胞化学染色,均显示阳性。
Nestin or 5-bromodeoxyuridine positive staining cells were found in suspension cultured and adhere-wall cultured human and rat neural stem cells.
悬浮培养及贴壁培养的人和鼠神经干细胞,经抗巢蛋白及抗5 -溴脱氧尿嘧啶核苷免疫细胞化学染色,均显示阳性。
Nestin or 5-bromodeoxyuridine positive staining cells were found in suspension cultured and adhere-wall cultured human and rat neural stem cells.
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