方法：采用PCR，克隆及连续亚克隆，序列分析，原核温度诱导表达等方法。
Methods: PCR, gene cloning and successive sub cloning, DNA sequencing, prokaryotic temperature induction, etc. were used.
融合蛋白表达量的高低与诱导时的细胞密度，诱导的温度以及培养基有关。在一定范围内与诱导剂的剂量以及诱导时间的长短无关。
The expression level was closely related with the cell density, induction temperature and medium but not with the inductor dosage and the induction period within certain range.
通过对表达载体的SDS-PAGE各种参数包括时间、温度和IPTG 浓度诱导表达分析，结果显示最佳诱导时间为3～4小时；
Through induced expressing and analyzing on various kinds of SDS-PAGE parameter including time, temperature and IPTG density, the result showed that the best induced time was 3~4 hours;
方法：通过实验条件的优化确定了适合GM-CSF表达的诱导温度、诱导时间和培养基等。
Methods:The experiment condition was optimized to determine the suitable inducement temperature, time and media of CM-CSF, etc.
将重组质粒转化至大肠杆菌宿主中，对目的蛋白进行诱导表达，并对诱导表达条件如温度、IPTG浓度等进行了优化。
The expression condition of plasmid pET-tmcC has been explored, but at all the testing temperature, IPTG concentration and different hosts protein expression was not found.
将重组质粒转化至大肠杆菌宿主中，对目的蛋白进行诱导表达，并对诱导表达条件如温度、IPTG浓度等进行了优化。
The expression condition of plasmid pET-tmcC has been explored, but at all the testing temperature, IPTG concentration and different hosts protein expression was not found.
应用推荐