方法:用混合酸酐法合成。
以辣根过氧化物酶采用改良的过碘酸钠法标记抗体、混合酸酐法标记半抗原。
The horseradish peroxidase (HRP) tagged antibody was prepared by means of modified NaIO 4 method and the HRP tagged hapten was prepared by means of mixed anhydride method.
以混合酸酐法将DES - MCPE与载体蛋白偶联形成完全抗原,并以紫外扫描分析了完全抗原的偶联比率。
The conjugates of DES-MCPE with proteins as complete antigens were prepared by the mixed anhydride method and the conjugation rate of them were analyzed by UV scanning.
由N-保护的精氨酸、C-保护的甘氨酸采用混合酸酐法缩合成RG二肽,再与天冬氨酸在液相中合成RGD三肽。
The RG Dipeptides were condensated from N-protected Arg and C-protected Gly by mixed anhydrides method. Then Asp and RG Dipeptides were synthesized RGD Tripeptides in liquid phase.
由N -保护的精氨酸、C -保护的甘氨酸采用混合酸酐法缩合成RG二肽,再与天冬氨酸在液相中合成RGD三肽。
The RG Dipeptides were condensated from N-protected Arg and C-protected Gly by mixed anhydrides method. Then Asp and RG Dipeptides were used synthesize RGD Tripeptides in liquid phase.
传统的肽类合成多采用液相化学合成,常用的包括碳二亚胺法,混合酸酐法、酰氯法、叠氮物法等等,为多肽的合成提供了多种选择。
Peptides are traditionally synthesized in liquid phase, such methods are DCC, mixed anhydrides, acyl chloride and azide etc. , which provide more choices for peptides synthesis.
方法以N -保护的色氨酸、甲硫氨酸、天门冬氨酸和C -保护的苯丙氨酸为原料,采用混合酸酐法合成了CCK四肽,总收率38.5%。
METHODS The CCK-4 peptides were condensed from N-protected Trp, met, Asp and C-protected Phe by Mixture anhydrides method, resulting in total yield of 38.5%.
方法以N -保护的色氨酸、甲硫氨酸、天门冬氨酸和C -保护的苯丙氨酸为原料,采用混合酸酐法合成了CCK四肽,总收率38.5%。
METHODS The CCK-4 peptides were condensed from N-protected Trp, met, Asp and C-protected Phe by Mixture anhydrides method, resulting in total yield of 38.5%.
应用推荐