探讨应用活细胞荧光染料CFSE 检测T淋巴细胞各亚群增殖反应的方法学。
Evaluate the method of determining the proliferation of human T lymphocyte subpopulations by using carboxyfluorescein diacetate succinimidyl ester labeling assay.
方法检测128例患者外周血t淋巴细胞亚群和免疫球蛋白水平，并观察急性期与恢复期的动态变化。
Method the levels of peripheral blood subgroup of t lymphocyte and immunoglobulin in 128 patients were investigated and their variation in acute and recovering stage of disease.
并检测部分患儿治疗前后的T -淋巴细胞亚群及血浆干扰素水平的变化。
The changes of T-lymphocyte subpopulation and the levels of plasma interferon (IF) were detected before and after treatment in some children.
应用T淋巴细胞亚群单克隆抗体对急性白血病患者外周血t淋巴细胞进行检测。
T lymphocyte cell subsets in peripheral blood of acute leukemia patients were determined with t lymphocyte subsets monoclonal antibodies method.
应用流式细胞法检测所有研t淋巴细胞亚群和NK细胞。
Peripheral t - lymphocyte subsets and NK cells were examined by flow cytometric analysis.
方法：应用流式细胞技术分别检测健康对照组以及MARS人工肝治疗前后肝功能衰竭患者外周血中t淋巴细胞亚群的绝对计数值，观察其变化规律。
Methods: to detect peripheral blood t lymphocyte adn in the patients with liver failure treated with MARS and the normal control by flow cytometer, observer the regular pattern was observed.
目的应用抗体包被免疫磁珠分离CD4 +T淋巴细胞亚群，并检测其效果。
Objective To isolate CD4+ T lymphocyte subpopulation by antibody-coating immunomagnetic beads and evaluate the efficacy of detective methods.
方法：用免疫组织化学方法检测循环血t淋巴细胞亚群。
Methods: Peripheral t lymphocyte subpopulations were estimated by immunohistochemical method of alkaline phosphatase.
目的探讨应用流式细胞术和活细胞荧光染料CFSE检测T淋巴细胞各亚群增殖反应的方法学。
Objective To evaluate the method of determining the proliferation of human T lymphocyte subpopulations by using flow cytometry and carboxyfluorescein diacetate succinimidyl ester(CFSE) labeling assay.
对60例荨麻疹患者用流式细胞仪检测外周血T淋巴细胞及其亚群并与20例健康志愿者作对照。
The peripheral blood T lymphocytes and their subsets in 60 patients with urticaria were detected by flow cytometry and compared with 20 healthy controls.
方法：在发病过程中抽取静脉血进行血常规、血生化指标以及T淋巴细胞亚群以及其他感染性指标的检测。
Methods The blood routine, biochemical test, T lymphocell subgroup as well as other infection marking in peripheral vein blood were detected in disease course.
结果VC患儿外周静脉血多项免疫指标值均低于对照组，其中以T淋巴细胞亚群、免疫球蛋白检测值差异有极显著性意义。
Results the immune values of the children with VC were lower than that of the control group. Especially, the t lymphocyte subgroup and immunoglobulin had significant statistic difference.
方法选择临床确诊的吉兰-巴雷综合征患者进行临床分期与分型，并用流式细胞仪检测其外周血T淋巴细胞亚群的相对计数。
Methods The clinical stage and clinical typing of patients with GBS were observed; T lymphocyte subsets in peripheral blood of patients with GBS were counted by flow cytometry.
目的：探讨联合检测血清免疫抑制酸性蛋白(iap)、T淋巴细胞亚群(TLS)、自然杀伤细胞(NK,CD 16 +56 +)、淋巴细胞转化(LTT)在临床的应用价值。
Objective: To explore the clinical values of detecting the immunosuppressive acid protein (IAP). T lymphocyte subgroup (TLS), nature Killer (NK, CD16 + 56 +) and lymphocyte transformation test (LTT).
方法：用流式细胞仪检测30例根治切除的肺癌患者术前、术后不同时期外周血淋巴细胞亚群中各种细胞因子。
Method:The peripheral blood lymphocyte subsets were examined in 30 patients with lung cancer before and after operation by flow cytometric analysis.
方法采用间接免疫荧光法检测白血病患者T淋巴细胞亚群、NK细胞百分数，MTT法检测NK细胞杀伤活性。
Methods t lymphoid subgroups and the percentage of NK cells were detected by the use of indirect immunofluorescence method the function of NK cells was detected by MTT.
用流式细胞仪检测21例儿童臀肌挛缩症患者外周血T、B淋巴细胞及其亚群，并以20名健康儿童作对照。
The peripheral blood T; B-lymphocytes and its subsets were analyzed by flow cytometry in 21 children with gluteal muscle contracture and 20 normal controls.
方法：对60例荨麻疹患者用流式细胞仪检测外周血t淋巴细胞及其亚群，并与40例健康志愿者作对照。
Methods: T-lymphocytes subpopulations of the peripheral Mood ire 60 patients with urticaria and 40 healthy controls were examined by flaw cytometry.
采用流式细胞仪进行检测，观察患者组治疗前后与正常对照组外周血T淋巴细胞亚群的变化情况。
The changes of T lymphocyte subsets in peripheral blood from 30 patients with acute psoriasis vulgaris and healthy controls were detected by using flow cytometry.
给药10天后处死小鼠，检测小鼠体重、脾脏和胸腺指数、血像、骨髓像、脾脏病理和T淋巴细胞亚群等各项指标的改变。
The mice were killed after 10 days and the mice's body weight, spleen and thymus index, hemogram, myelogram, pathological changes of spleen, t lymphocyte subpopulation were detected.
目的通过对不同肝病患者与正常对照组外周血清免疫球蛋白及T淋巴细胞亚群的检测，探讨肝病患者的机体免疫状态及临床意义。
Objective\ To evaluate the clinical implications of serum immunoglobulins and peripheral T-lymphocyte subsets in patients with various hepatic diseases.
目的通过对不同肝病患者与正常对照组外周血清免疫球蛋白及T淋巴细胞亚群的检测，探讨肝病患者的机体免疫状态及临床意义。
Objective\ To evaluate the clinical implications of serum immunoglobulins and peripheral T-lymphocyte subsets in patients with various hepatic diseases.
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