方法从狗的胸导管分离和培养淋巴管内皮细胞。
Methods Lymphatic endothelial cells from canine thoracic ducts were isolated and incubated.
方法用狗的胸导管分离和培养淋巴管内皮细胞。
Methods the lymphatic endothelial cells of the canine thoracic ducts were isolated and incubated.
在内皮细胞表达，是血管和淋巴管内皮细胞标记物。
Theendothelial marker CD31(PECAM-1) is expressed by endothelium of both lymphatic and blood vessel.
材料和方法本实验所用淋巴管内皮细胞来自猪的胸导管。
Materials and Methods LECs were taken from the pig thoracic duct.
前言：目的：建立分离猪淋巴管内皮细胞的技术及其培养方法。
Objective: To establish the technology of isolation of Lymphatic endothelial cells of pig thoracic duct and the culture method of their endothelium.
巨噬细胞与淋巴管内皮细胞的彼此接触面缺乏紧密连接和缝隙连接等连接形式。
The contact point between the macrophage and lymphatic endothelium was deficient of junctions such as tight junction and gap junction.
结果(1)淋巴管内皮细胞的形态学特征：内皮细胞呈扁平、短梭形或多角形。
Results (1) Morphological characteristics of LEC: They were flat, elongated or polygonal shape.
目的探讨淋巴管内皮细胞的培养技术与鉴定方法，为开展有关方面的研究提供技术支持。
ObjectiveTo study the culture technique and differentiation method of lymphatic endothelial cells, and supply support for the related research of this field.
采用划线法和MTT法观察血管生成抑制素和反应停对淋巴管内皮细胞增殖和游走的影响。
Scraping line method and MTT were used to observe the effect of angiostatin and thalidomide on proliferation and migration of LEC.
目的探讨淋巴管内皮细胞透明质酸受体-1染色阳性（LYVE-1+）淋巴管在大鼠肾内的分布。
Objective To investigate the distribution of intrarenal lymphatic vessels in developing rat using the lymphatic marker LYVE-1.
在培养的细胞中，采用VEGFR - 3和LYVE - 1因子鉴定淋巴管内皮细胞的方法可靠。
It was reliable to use VEGFR-3 and LYVE-1 to identify the lymphatic endothelial cells.
结果正常大肠组织内未见VEGF - C阳性表达，但可见淋巴管内皮细胞VEGFR - 3阳性表达。
Results There was no expression of VEGF-C in normal colorectal tissues, but VEGFR-3 expressed in the lymphatic endothelium.
目的比较淋巴管内皮细胞对乳腺癌和骨肉瘤生长和转移的影响，分析淋巴管生成对肿瘤生长和转移影响的相关机制。
Objective To compare growth and metastasis of breast cancer cell and osteosarcoma cell influenced by co-inoculating human lymphatic endothelial cell (HLyEC), and to analyze its mechanisms.
VEGF-C主要通过其受体VEGFR-2、VEGFR-3发挥作用，是一种重要的血管、淋巴管内皮细胞调节因子。
VEGF-C and its receptor VEGFR-2/VEGFR-3 are the key players in the molecular regulation of lymphangiogenesis.
结果VEGFR- 3非特异地表达于微血管和淋巴管内皮细胞浆，而LYVE - 1仅特异地表达于淋巴管内皮细胞浆中。
Results VEGFR-3 was expressed in both lymphatic vessel endothelial and vessel endothelial cytoplasm, but LYVE-1 only in lymphatic vessel endothelial cytoplasm.
目的研究血管内皮生长因子C（VEGF C）对于淋巴管内皮细胞增殖和迁移的作用，探讨VEGF C促进淋巴管新生的机制。
Objective To investigate effects of VEGF C on proliferation and migration of lymphatic endothelial cells and explore mechanism of lymphangiogenesis induced by VEGF C.
在淋巴管铸型上，清晰地显示出具有特征性的办膜切迹，相当淋巴管双瓣膜所在的位置。此外，在铸型表面还可以见到淋巴管内皮细胞核的压迹。
The distinct imprints which correspond to the bicuspid valves of lymphatics and the oval or fusiform impressions of the endothelial nuclei were seen on the casts.
结论：VEGF - C通过介导淋巴管内皮细胞及淋巴管增殖有利于颈淋巴道转移的发生，VEGF - C检测可作为预测淋巴道转移及预后判断的指标之一。
Conclusion: These findings suggest that VEGF-C may promote lymphatic metastasis of oral cancer, and it is possible to be used as a predictor for the lymphatic metastasis and prognosis of oral cancer.
结论：VEGF - C通过介导淋巴管内皮细胞及淋巴管增殖有利于颈淋巴道转移的发生，VEGF - C检测可作为预测淋巴道转移及预后判断的指标之一。
Conclusion: These findings suggest that VEGF-C may promote lymphatic metastasis of oral cancer, and it is possible to be used as a predictor for the lymphatic metastasis and prognosis of oral cancer.
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