方法以体外培养雌激素受体阳性细胞MCF - 7为研究对象，采用MTT法和流式细胞术法检测MCF - 7细胞存活率和细胞凋亡。
Methods MTT assay and flow cytometer (FCM) assay were used to determine the survival rate and apoptosis of MCF-7 cells, which was estrogen receptor positive in culture.
利用磁吸附细胞分选法(MACS)分离大鼠脾脏CD 4 +T细胞，流式细胞术检测MACS分选前后CD 4 + T细胞比例，监测细胞分选效果。
Magnetic absorption cell sorting (MACS) method was used to isolate the CD4 + t lymphocytes from the spleen of the rats. Flow cytometry was used to monitor the efficiency of isolation.
分别采用台盘蓝拒染法及流式细胞术，在不同条件下测定细胞增殖的抑制率及细胞周期的分布。
Methods The cell growth was tested by trypan blue stain assay and the cell cycle was analysed by flow cytometry in the various conditions.
方法随机选择血常规标本20例，用流式细胞术测定外周血循环内皮细胞，并与手工法比较。
Methods Flow cytometry and manual counting were applied to measure circulating endothelial cells in 20 blood routine samples.
结论流式细胞术在循环内皮细胞检测中优于手工法。
Conclusion the efficiency of flow cytometry was superior to manual counting in measuring circulating endothelial cells.
方法：ABC免疫组化法及流式细胞术。
Methods: ABC immunohistochemical technique and flow cytometry were used.
方法：用液体或半固体培养法及流式细胞术测定血小板因子4对CD34阳性脐血细胞的增殖与分化的作用。
Methods: Liquid and semi solid culture systems as well as flow cytometry were used to study the effect of PF4 on the proliferation and differentiation of CD34+ cord blood cells.
方法利用免疫细胞化学，蛋白质印迹法，RT -PCR和流式细胞术等方法分析细胞周期和对与细胞周期相关的基因表达进行研究。
Methods the cell cycle and the expression of related genes were analyzed by the methods of immunocytochemistry, protein blotting, RT PCR and flow cytometry.
方法应用MTT法检测不同浓度的5-氮-2’-脱氧胞苷对胆管癌细胞株QBC939存活率的影响，应用流式细胞术检测5-氮-2’-脱氧胞苷对QBC939细胞生长周期及凋亡率的影响。
Methods The survival rate and apoptosis rate of QBC939 after being treated with different density of 5-aza-2'-deoxycytidine were detected by using the method of MTT and flow cytometry.
方法：四氮唑蓝比色法(MTT法)观察梁金菇多糖对HL 6 0细胞的抑制率，应用形态学、流式细胞术、DNA凝胶电泳等方法检测细胞凋亡的发生。
METHODS: The proliferation inhibition effects of LJPS on HL-60 cells was observed by MTT colorimetry. Apoptosis was detected by morphology, flow cytometry and DNA agarose gel electrophoresis.
方法采用酶直接消化法从胎盘组织中提取间充质干细胞，并利用流式细胞术及染色体核型分析技术鉴定其干细胞特性。
Methods the enzyme directory digestion method was used to isolate Mesenchymal stem cells from Human Placenta and flow cytometry and karyotyping were used to evaluated the character of stem cells.
方法：应用形态学观察，MTT分析法，DNA梯状电泳，流式细胞术检测术，研究紫杉醇对成骨肉瘤细胞系SO SP- 96 0 7的细胞毒效应。
Methods: The cytotoxic effect of paclitaxel on human osteosarcoma cell line SOSP-9607 was examined by MTT assay, morphological observation, gel electrophoresis of DNA, and cell morphology.
方法：应用形态学观察，MTT分析法，DNA梯状电泳，流式细胞术检测术，研究紫杉醇对成骨肉瘤细胞系SO SP- 96 0 7的细胞毒效应。
Methods: The cytotoxic effect of paclitaxel on human osteosarcoma cell line SOSP-9607 was examined by MTT assay, morphological observation, gel electrophoresis of DNA, and cell morphology.
应用推荐