方法应用免疫细胞化学和流式细胞术方法。
Methods The immunocytochemical and flow cytometry were used.
方法采用流式细胞术对尚未进行化疗的91例恶性肿瘤患者体腔液细胞DNA含量进行了检测和分析。
Methods DNA contents in coelom fluid cells were measured and analyzed from 91 patients with malignant carcinomas by flow cytometry.
方法采用三色免疫荧光直接标记法及多参数流式细胞术分析13例多发性骨髓瘤的免疫表型。
Methods Three-colour direct immunofluorescence staining and multiparameter flow cytometry were used for analysis of 13 samples of immunophenotyping of multiple myeloma patients.
方法骨髓有核细胞免疫荧光标记后用流式细胞术分析。
Methods Bone marrow nuclear cells (BMNCs) were labeled by immunofluorescence and analyzed by flow cytometry.
运用鉴别细胞群体生物学特性的常规方法和流式细胞光度术(FCM)，我们研究了正了酸对人肺癌细胞(PLA- 801d)的某些生物学作用。
The effects of butyric acid on cloned human lung cancer cells (PLA-801D) were observed with flow cytometry (FCM) and the conventional methods to identify the biological characteristics of cell lines.
方法整群抽取桑拿浴服务人员外周血196例，采用流式细胞术观察T淋巴细胞免疫表型。
Methods to get 196 blood samples by using cluster sampling in sauna bath. To observe t lymphocyte immunophenotype with flow cytometer.
目的：应用流式细胞术，建立一种准确、外周血嗜酸性细胞及其相关分子的快速测定方法。
AIM: to set up an accurate and rapid method to detect the phenotype of peripheral blood eosinophils using flow cytometry.
方法：采用间接免疫荧光流式细胞术，分析K562细胞表面TRAIL分子的表达。
METHODS: The expression of TRAIL on K562 cells was detected by indirect immunofluorescence staining and flow cytometry.
方法：ABC免疫组化法及流式细胞术。
Methods: ABC immunohistochemical technique and flow cytometry were used.
方法取新鲜NSCLC标本28例，应用流式细胞术测定CD44，同时测定凋亡细胞峰，并与癌旁肺组织进行比较。
Methods With flow cytometry, expression of CD44 and apoptosis cell peak were detected in 28 fresh specimens of NSCLC and normal pulmonary specimens.
方法采用流式细胞术和特异性标记单克隆抗体，测定内皮细胞表面黏附分子VCAM - 1及CD62E的表达率。
Methods the expression rates of endothelial cell surface adhesion molecules VCAM-1 and CD62E were detected by flow cytometry (FCM) and specific labeled monoclonal antibody.
方法：采用间接免疫荧光标记，流式细胞术分析。
Methods Indirect immune fluorescent labeling and flow cytometry were employed.
方法用流式细胞术测定165例恶性肿瘤病人的CD25、CD56的结合表达率。
Methods The expression rates of the CD25CD56 in 165 malignant tumor patients were examined by using flow cytometry.
目的建立流式细胞术检测循环全血中单核细胞与血小板聚合率的方法。
Objective to establish a method for detection of circulating monocyte-platelet aggregates by flow cytometry.
方法采用多色流式细胞术结合血液分析仪测定38例SARS患者外周血淋巴细胞及其亚群的表达情况。
Method Flow cytometer and hematology analyzer were used to detect the expression of lymphocytes and its subsets in 38 patients of SARS.
方法：通过建立免疫缺陷模型，经复方活性多糖干预，采用流式细胞术观察t细胞亚群等变化。
Methods: We observe the change of the t lymphocyte subgroup and so on through flow cytometer by building a immunodeficiency model with compound amylose's intervention.
方法：用液体或半固体培养法及流式细胞术测定血小板因子4对CD34阳性脐血细胞的增殖与分化的作用。
Methods: Liquid and semi solid culture systems as well as flow cytometry were used to study the effect of PF4 on the proliferation and differentiation of CD34+ cord blood cells.
方法应用流式细胞术分析人胃癌MGC803细胞在DADS处理前后细胞周期分布的变化。
Methods cell cycle of MGC803 cell treated with DADS in vitro was analyzed by flow cytometry.
方法采用流式细胞术检测31例apl患者的免疫表型。
Methods Immunophenotype was examined by flow cytometry in 31 patients with APL.
方法随机选择血常规标本20例，用流式细胞术测定外周血循环内皮细胞，并与手工法比较。
Methods Flow cytometry and manual counting were applied to measure circulating endothelial cells in 20 blood routine samples.
目的探讨应用流式细胞术和活细胞荧光染料CFSE检测T淋巴细胞各亚群增殖反应的方法学。
Objective To evaluate the method of determining the proliferation of human T lymphocyte subpopulations by using flow cytometry and carboxyfluorescein diacetate succinimidyl ester(CFSE) labeling assay.
方法利用免疫细胞化学，蛋白质印迹法，RT -PCR和流式细胞术等方法分析细胞周期和对与细胞周期相关的基因表达进行研究。
Methods the cell cycle and the expression of related genes were analyzed by the methods of immunocytochemistry, protein blotting, RT PCR and flow cytometry.
方法：应用流式细胞术检测80例大肠癌标本中CD34的表达。
Methods: Flow cytometry (FCM) was used to detect the expression of CD34 in 80 cases cancer of colon.
方法应用流式细胞术检测了35例急性淋巴细胞性白血病患者骨髓细胞DNA含量(DI)、倍体和细胞增殖活性(PI)。
Methods The DNA index (DI) and proliferation index (PI) in aspirated myeloid cells were determinated in 35 patients with ALL by flow cytometry (FCM).
方法应用流式细胞术和细胞免疫荧光染色技术。
Methods It was studied by flow cytometry and immunofluorescence.
方法以体外培养雌激素受体阳性细胞MCF - 7为研究对象，采用MTT法和流式细胞术法检测MCF - 7细胞存活率和细胞凋亡。
Methods MTT assay and flow cytometer (FCM) assay were used to determine the survival rate and apoptosis of MCF-7 cells, which was estrogen receptor positive in culture.
方法：采用细胞免疫荧光染色技术和流式细胞术。
Methods Flow cytometry and immunofluorescence staining technique were used.
方法：应用基因芯片、反转录PCR（RT-PCR）、流式细胞术等方法从分子和蛋白水平检测两种细胞系中CXCR4的表达情况。
Methods: Gene chip and RT-PCR were used to study expression of CXCR4 mRNA in two cell lines and the CXCR4 surface expression was also examined by flow cytometry analysis.
方法采用流式细胞术。
方法采用流式细胞术。
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