方法：应用荧光标记单克隆抗体和流式细胞技术。
METHODS: Using fluorescent-labeled monoclonal antibodies and flow cytometry.
方法：用流式细胞技术(FCM)检测G蛋白亚型的变化。
Methods: The level of G protein subunits in RPMVECs membrane was examined by flow cytometry (FCM).
用流式细胞技术检测各组细胞周期，观察细胞增殖能力的变化。
The changes in cell cycle progression of synchronized HUVECs were examined by flow cytometric analysis.
用间接免疫荧光染色结合流式细胞技术检测细胞表面DR4和DR5分子表达；
The expression of cell surface-bound DR4 and DR5 were determined by indirect fluorescence staining and flow cytometry analysis.
方法采用流式细胞技术(FCM)用三色标记法检测脑损伤患者治疗前后CD 28的表达。
Methods the levels of CD28 of the patients of craniocerebral trauma before and after treatment were detected by flow cytometry (FCM).
进而，采用流式细胞技术研究了不同蛋白酶体抑制剂以及TAP对噬菌体颗粒抗原交叉呈递的影响。
Furthermore, the effect of proteasome inhibitors and TAP on particle antigens cross presentation was investigated by FACS analysis.
方法：实验分人参皂甙组、对照组和GM -CSF组，采用流式细胞技术测定各组细胞的凋亡率。
Methods: the experimental subjects were divided into GS group, control group and GM-CSF group. FACs technology had been used to determine the apoptosis ratio.
应用流式细胞技术研究机械拉伸应变对成骨细胞增殖的影响，探讨此类细胞在力学生理背景下的生长行为。
To determine the proliferative effect of mechanical stretching on osteoblasts, cell cycle analysis was carried out by flow cytometry.
方法分别采用流式细胞技术和散色比浊法测定HSP急性期与恢复期及对照组儿童血清免疫球蛋白浓度和T淋巴细胞亚群水平。
Methods The immunoglobulin concentration and T-cell subgroup level of acute stage and convalescent stage HSP children were detected with FCM and nephelometer.
方法利用盐酸利多卡因注射液联合震荡培养法分离纯化胎大鼠脑内小胶质细胞，免疫荧光技术及流式细胞技术鉴定小胶质细胞的纯度。
Method: Fetal rat microglia in brain was isolated and purified by lidocaine hydrochloride with shock culturing. The purification of microglia was detected by immunofluorescence and flow cytometry.
方法采用LRP单克隆抗体、流式细胞技术分别测定15例单纯缺铁性贫血患者（对照组）和65例AL患者（AL组）LRP的表达率。
The expression rate of LRP was detected by its monoclonal antibody and flow cytometry in 15 asiderotic anemia patients and 65 AL patients.
方法：应用流式细胞技术分别检测健康对照组以及MARS人工肝治疗前后肝功能衰竭患者外周血中t淋巴细胞亚群的绝对计数值，观察其变化规律。
Methods: to detect peripheral blood t lymphocyte adn in the patients with liver failure treated with MARS and the normal control by flow cytometer, observer the regular pattern was observed.
方法：采用流式细胞仪单染色和三色荧光标记技术。
Methods: to use mono color and three color fluorescent staining methods by flow cytometry.
方法运用流式细胞仪三色分析技术检测25例不稳定型心绞痛患者在发病期和缓解期以及20名正常健康对照外周血标本cd 28、CD4的表达。
Methods The expression of CD28 and CD4 in peripheral blood of 20 healthy persons and 25 patients with UAP was detected by triad colour analytical technique with flow cytometry.
并通过流式细胞仪技术对其进行鉴定。
Using the flow cytometry technology to judge whether the cell is MSCs.
方法应用流式细胞术和细胞免疫荧光染色技术。
Methods It was studied by flow cytometry and immunofluorescence.
用TUNEL技术和流式细胞仪技术测定了COPD患者外周血PMN和AM的凋亡变化及其规律。
The dynamics changes of apoptotic polymorphoneuclear(PMN) and AM in COPD patients were measured by flow cytometry and TUNEL techniques.
方法采用流式细胞仪技术对不同培养时间和不同培养条件下脐血cd 34 +细胞中pcna的表达水平和细胞周期进行了测定。
Methods Using flow cytometry, PCNA and cell cycle in CB CD34 + cells were measured in different conditions and different culture time.
方法：采用荧光显微镜、电子显微镜、激光扫描共聚焦显微镜、流式细胞分光光度计和DNA凝胶电泳等方法和技术观察细胞凋亡。
Method: Using fluorescence microscopy, electron microscopy, laser scanning confocal microscopy, flow cytometry and DNA gel electrophoresis to demonstrate the presence of apoptosis.
方法：采用逆转录聚合酶链反应(RT - PCR)、流式细胞仪和免疫荧光细胞染色分析技术分析肺上皮细胞系a549细胞PAR s的表达情况。
METHODS: We used RT-PCR, flow cytometry and immunofluorescence cell staining techniques to observe the expression of PARs on A549 cells.
方法用流式细胞仪检测缺血缺氧后不同时间点星形胶质细胞细胞周期变化，并用荧光免疫细胞化学技术测定胶质细胞纤维酸性蛋白（GFAP）和增殖细胞核抗原（PCNA ）的表达水平。
Methods We measured the astrocyte cell cycles in different time after ischemia and anoxia by flow cytometry and detected the levels of GFAP and PCNA with fluorescence immunocytochemistry.
并用TUNEL技术和流式细胞仪检测了相关淋巴细胞的凋亡。
TUNEL and flow cytometry were used to detect apoptotic cells.
并通过流式细胞仪和激光共聚焦显微镜等技术探讨了聚合物在细胞水平的靶向能力及靶向机理。
As revealed by flow cytometry and confocal microscopy, the dendrimer-biotin conjugate exhibits exhibited much higher cellular uptake into cancer cells than the conjugate without biotin.
方法采用MTT法和流式细胞仪技术。
结果①流式细胞术分选后共聚焦显微镜分析技术（PSC）能同时、准确地鉴别不同细胞周期时相的凋亡细胞和非凋亡细胞。
Results Post-sorting confocal (PSC) method could exactly distinguish apoptotic and non-apoptotic cells in the specific cell cycle phases at the same time.
流式细胞仪(FCM)是综合了多项现代技术的科学仪器，在生物学、基础医学和临床医学获得了广泛应用。
Flow cytometry (FCM) which colligated a few of modern techniques is widely used in biology, basic medicine and clinical medicine.
结论胞质抗体具有系列特异性，四色组合流式细胞-免疫表型技术能提供快速多参数的分析数据。
Conclusion Intracellular antibodies possess lineage specificity and four-color combination flow cytometric immunophenotyping can provide fast and multi-parameter data.
采用硝酸甘油偏头痛模型大鼠，运用透射电镜和流式细胞仪技术进行研究。
Methods To choose disease model of migraine with nitroglycerin by means of flow cytometry and transmission electron microscope.
方法采用酶直接消化法从胎盘组织中提取间充质干细胞，并利用流式细胞术及染色体核型分析技术鉴定其干细胞特性。
Methods the enzyme directory digestion method was used to isolate Mesenchymal stem cells from Human Placenta and flow cytometry and karyotyping were used to evaluated the character of stem cells.
方法：采用细胞免疫荧光染色技术和流式细胞术。
Methods Flow cytometry and immunofluorescence staining technique were used.
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