目的构建携带双自杀基因且可诱导敲除SV40T的逆转录病毒载体，优化目前的肝细胞永生化。
Objective To construct a new retroviral vector with double-suicide genes and knockout-inducible SV40T gene, with the aim of optimizing immortalization of hepatocytes.
潜伏或永生化感染可以在DNA病毒感染的细胞中引起，因其限制或缺乏病毒基因转录所需的设备材料。
A latent or immortalizing infection may result from DNA virus infection of a cell that restricts or lacks the machinery for transcribing all the viral genes.
每一条基因都渴望永生。
每一条基因都渴望永生。
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