目的:对牛牙骨质附着蛋白进行N末端氨基酸序列分析,为牙骨质附着蛋白的克隆表达提供一定的理论依据。
Objective:To analyze the N-terminal amino acid sequence of bovine cementum attachment protein(CAP), which lay a theoretical foundation for cloning and expression of the CAP.
方法:用基因测序、氨基酸组成分析、N末端氨基酸测序、胰肽图谱、IEF、分子量、紫外扫描等各种实验手段对重组L 天冬酰胺酶及其天然品进行了全面比较。
Methods:Various analytical techniques including gene sequencing, amino acid composition, analysis of N terminal amino acid analysis, peptide mapping, IEF, molecular weight and UV scanning were used.
方法:用基因测序、氨基酸组成分析、N末端氨基酸测序、胰肽图谱、IEF、分子量、紫外扫描等各种实验手段对重组L 天冬酰胺酶及其天然品进行了全面比较。
Methods:Various analytical techniques including gene sequencing, amino acid composition, analysis of N terminal amino acid analysis, peptide mapping, IEF, molecular weight and UV scanning were used.
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