由下胚轴成功的诱导出毛状根。
目的：建立人参毛状根转化体系。
从毛状根上直接分化出不定芽和根。
毛状根能在不含激素的MS培养基上生长。
前言： 目的： 诱导川黄柏毛状根的发生。
Objective: To introduce the hairy roots of Phellodendron chinense and determine the content of its active constituents.
目的：研究人参毛状根生物合成熊果苷的基本条件。
Objective: To study basic conditions for biosynthesis arbutin by the hairy root of Panax ginseng .
毛状根培养是生产植物次生代谢产物的新途径、新方法。
Hairy roots culture is a new means to gain the plant secondary metabolites.
培养基的电导率变化与毛状根的生物量积累呈线性相关。
The medium conductivity decreased linearly relative to the biomass accumulation.
毛状根的诱导频率与无菌苗培养天数和外植体来源部位有关。
The frequency of hairy root induction was closely related to the age of seedlings and explant resources.
本文综述了发根农杆菌诱导植物毛状根的方法及其分子机制。
The method of hairy root induction of plants as well as their molecular biology are reviewed.
利用超声雾化生物反应器进行青蒿毛状根多层培养生产青蒿素。
A ultrasonic mist bioreactor was used to produce artemisinin by Artemisia annuaL.
目的：研究丹参毛状根对麻醉大鼠心肌缺血再灌注损伤的保护作用。
The protection on myocardial ischemia and reperfusion injury in intact rats by Salvia miltiorrhiza hairy root was investigated.
目的：考察真菌诱导子对人参毛状根的生长和人参皂苷生物合成影响。
Objective: to study influence of fungal elicitors on the biomass and ginseng saponin biosynthesis of hairy roots of Panax ginseng (HRPG).
利用高压纸电泳法对毛状根和再生植株进行T-DNA转化的检测。
The transformation of Ri T DNA was examined through high voltage paper electrophoresis.
检测结果表明，毛状根中的总生物碱含量高于长春花的原植株和愈伤组织。
The analysis result showed that the contents of total alkaloids in hairy roots were higher than explants and calli.
添加无水乙醇能够有效促进三分三毛状根生物碱的合成，使其生物碱含量显著提高；
EtOH was added showed a drastically increase of alkaloid content, particularly scopolamine was significantly increased compared with the control cultures.
毛状根的龙胆苦甙含量(2.25%)接近母本野生龙胆根的含量(3. 43%)。
The hairy roots content of gentiopicroside (2. 25 %) was close to the roots of wild maternal plant (3. 43 %).
结论 ：金铁锁毛状根离体培养系统的成功建立，对进一步规模化培养金铁锁毛状根具有重要意义。
Conclusion : Establishment of hairy roots culture of P. tunicoides provided a foundation for industrial production of active components from P. tunicoides culture.
蓝光和白光下培养的三裂叶野葛毛状根的表面呈淡绿色，而暗培养的三裂叶野葛毛状根呈淡红褐色或白色。
When cultured in the white light and blue light, the hairy roots of P. phaseoloides looked light green, but white or light red-brown in the dark.
而大量元素中含量过多的NH4NO3不利于丹参毛状根的生长，同时也不利于丹参酮类成分的积累和释放。
However, in the macroelements excessive NH4NO3 does not favor the growth of hairy root, the accumulation and release of tanshinones.
结果：高压纸电泳结果显示，所获毛状根中均含有甘露碱，表明毛状根中确已转入发根农杆菌的T-DNA；
Results: The results of paper electrophoresis showed that the hairy roots contained mannopine, this evidenced the integration of Ri T DNA in the transformed plants;
本文概述了培养毛状根生产药物研究的新成果和存在的问题，为进一步开发利用毛状根提出了可供参考的建议。
This review deals with recent development of hairy root cultures for pharmaceuticals and points out the future ways of hairy root cultures for pharmaceuticals.
结论：人参毛状根生长迅速，皂苷含量高于原药材，该体系的建立为利用生物技术工业化生产人参活性成分奠定了基础。
Conclusion: HRPG grows rapidly and proves high in content of ginseng saponin. This culture system of HRPG will be useful for the production of active components in ginseng.
主要从以下几个方面进行研究：离体培养体系的建立、遗传转化体系的建立、优良毛状根系的筛选、毛状根最佳培养条件的建立。
This study showed that in vitro systems establish, transformed systems establish, the choiceness hairy root lines select and the optimal culture condition of hairy roots systems establish.
ATCC 15834菌株诱导牛膝产生的毛状根可在无任何激素的MS培养基上快速生长，且具有分枝性强、丛生、无向地性等特点。
The hairy roots transformed by ATCC15834 grew rapidly on MS medium without any phytohormones, and were characterized by strongly branching, clustering and non-geotropism, etc.
ATCC 15834菌株诱导牛膝产生的毛状根可在无任何激素的MS培养基上快速生长，且具有分枝性强、丛生、无向地性等特点。
The hairy roots transformed by ATCC15834 grew rapidly on MS medium without any phytohormones, and were characterized by strongly branching, clustering and non-geotropism, etc.
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