植物原生质体因少了细胞壁更便于各种实验研究，自20世纪七十年代以来，已被学者们广泛的研究和应用。
Since the seventies of 20th century, the protoplast have been extensively studied and applied by scholars, as plant protoplasts can be used much more easily without cell wall.
原生质体是植物遗传改良的重要实验体系之一，而原生质体的分离是该体系的基础。
Protoplast is an important system for plant genetic improvement, and the isolation of protoplast is an essential step for the protoplast manipulation.
该培养基适于培养大豆等豆科植物未成熟子叶原生质体，通过胚性愈伤组织，经胚胎发生途径。
This callus can differentiate somatic embryo and regenerate plant on the culture medium somatic embryo differentiation.
对鹰嘴紫云英抗性系、半日花、霸王三种植物进行了原生质体分离和培养条件的研究。
The conditions of isolation and culture of protoplasts from resistant Astragalus cicer L. cell line, Helianthemum Songaricum Schrenk and Zygophyllum xanthoxylum were studied.
目前，原生质体培养在药用植物高产细胞系的筛选、体细胞杂交和基因工程方面已取得了长足的进展。
Recently, the protoplast culture of medicinal plants has mace great progress in the saltation of high productive cell lines, somatic cell hybridization and gene engineering.
还研究了两种植物愈伤组织的继代天数和不同的酶组合对原生质体游离的影响。
The effects of subculture days of 2 species and of different enzyme combinations on this dissociation process were also studied.
用纤维素酶(按照中国科学院上海植物生理研究所的方法制备)分离酒草叶肉细胞，获得大量完整的有活力的原生质体。
Usiug the cellulase "EA_3-867" prepared according to the method of the plant Physiology Institute of China, a large amount of vital and intact protoplasts are isolated from the Tobacco variety.
用纤维素酶(按照中国科学院上海植物生理研究所的方法制备)分离酒草叶肉细胞，获得大量完整的有活力的原生质体。
Usiug the cellulase "EA_3-867" prepared according to the method of the plant Physiology Institute of China, a large amount of vital and intact protoplasts are isolated from the Tobacco variety.
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