目的:发现新的检测沙眼衣原体的方法。
Objective:To find a new method to detect trachoma chlamydia.
实验室检测沙眼衣原体平均感染率22.2%。
方法采用聚合酶链反应技术(PCR)检测沙眼衣原体和解脲支原体的DNA。
Methods DNA of CT and UU was detected with polymerase chain reaction(PCR) technique.
目的探讨本地区性传播疾病(STD)患者沙眼衣原体(CT)、解脲支原体(UU)的感染现状及使用核酸扩增杂交梳法检测CT、UU的价值。
Objective To detect the status of CT and UU infection in local (STD)patients, and the value of measuring CT and UU with Nucleic Acid Amplification Hybridization Climb method.
目的:应用连接酶链反应(LCR)技术检测男性尿标本中的淋病奈瑟菌和沙眼衣原体,初步评价其敏感性和特异性。
Objective: To determine the sensitivity and specificity of ligase chain reaction(LCR) for the detection of Neisseria gonorrhoeae and Chlamydia trachomatis in the first-void urine of men.
采集328份肺炎新生儿鼻咽标本,分别对其进行培养和LCR扩增,应用ELISA检测有无沙眼衣原体感染。
Nasopharyngeal swabs taken from 328 neonates with pneumonia were analyzed by LCR-ELISA and cell culture. Results A method of LCR-ELISA for detection C. trachomatis infection was established.
结论荧光定量PCR法在检测沙眼体较金标法更敏感、快速,是早期诊断生殖道沙眼衣原体感染的一种极有价值的方法。
Conclusion: It is suggested that PCR in detecting chlamydozoa trachomatis appears to be more sensitive and more rapid than gold-labelled antigen detection method.
结论:滤纸条取液技术取样作沙眼衣原体的多聚酶链反应检测效果好。
The positive rates between different types of tubal pathological changes are not obviously different. Conclusions: The filter paper technique is an effective method in detecting CT-DNA by PCR.
方法采用聚合酶链反应(PCR)技术进行淋菌、沙眼衣原体、解脲脲支原体3种常见病原体检测。
METHODS Polymerase chain reaction(PCR) technology was taken to detect out Neisseria gonorrhoeae, Chlamydia trachomatis and Ureaplasma urealytium.
方法采用聚合酶链反应(PCR)技术进行淋菌、沙眼衣原体、解脲脲支原体3种常见病原体检测。
METHODS Polymerase chain reaction(PCR) technology was taken to detect out Neisseria gonorrhoeae, Chlamydia trachomatis and Ureaplasma urealytium.
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