密度梯度离心法分离肝内淋巴细胞；
The intrahepatic lymphocytes from each group were isolated by using density gradient centrifugation.
测滴度并用氯化铯梯度离心纯化备用。
The titer was assayed and CsC1 density gradient centrifugation and purification were applied.
采用蔗糖密度梯度离心法分离肝脏内质网。
Hepatic endoplasmic reticulum was isolated by differential centrifugation with sucrose density gradient.
密度梯度离心法体外培养骨髓间充质干细胞。
Methods Dental pulp stem cells were cultured in vitro, purified and then subcultured .
用改进的不连续蔗糖密度梯度离心法分离到五条带。
Five zones were separated by improved discontinuous sucrose gradient centrifugation.
方法不连续密度梯度离心法分离纯化大鼠肝贮脂细胞。
METHODS Rat lipocyte was isolated and purified with discontinuous density-gradient centrifugation.
大鼠心肌细胞核采用差速离心和密度梯度离心分离提纯。
Velocity and isopyknic gradient centrifugation was employed to fractionate rat myocardial nuclei.
结论通过密度梯度离心联合贴壁筛选可以获得脾脏epc。
CONCLUSION Density gradient centrifugation combined with adherence cells filtration is an effective way to obtain EPC derived from mice spleen.
梯度离心的分离方法增加细胞损伤，不能达到分离不同细胞的效果。
Separationby gradient centrifugation increased cell trauma and could not separate the different cells.
方法采用链霉蛋白酶消化、间断密度梯度离心纯化大鼠胃黏膜壁细胞。
Methods Rat gastric parietal cells were isolated by pronase digestion and purified by discontinuous density-gradient centrifugation.
方法用梯度离心和贴壁培养法分离msc，进行表面分化抗原的鉴定。
Methods Gradient centrifugation and adherent culture were used to get a great amount of NSC and then differentiation antigens were identified.
骨髓单个核细胞取自同系健康大鼠的四肢长骨，用密度梯度离心法获得。
BM MNCs were isolated from long bone of limbs in healthy Lewis rats by centrifugation through a histopaque density gradient.
本实验全面改进了用于蛋白质起始翻译阶段研究的传统介质梯度离心法。
The traditional sucrose gradient centrifugation for protein initiation translation analysis was improved greatly.
用密度梯度离心法分离并计数支气管肺泡灌洗液（BALF）中的嗜酸性粒细胞；
The eosinophils in broncho-alveolar lavage fluid (BALF) were separated by density gradient centrifugation.
目的比较免疫磁珠法和密度梯度离心法分离纯化人外周血中性粒细胞的可靠性。
Objective To compare the reliability of immunomagnetic beads and density gradient centrifugation in purification and isolation of neutrophilic granulocytes from human peripheral blood.
对蔬菜绿叶通过组织匀浆，差速离心分离，密度梯度离心分离、纯化、提取叶绿体。
The green leaves of vegetables through tissue homogenate, differential centrifugation separation, density gradient centrifugation purify sample of chloroplast.
用密度梯度离心法分离后MEM培养基体外培养的人胚胎msc纯度高，生物活性好。
The purity of Human embryonic MSC which isolated by density gradient centrifugation and cultured by MEM culture medium in vitro is high. The activity of MSC is good.
鉴于产品开发要求，试验研究确定选用蔗糖密度梯度离心方法作为流感疫苗的纯化手段。
As technological research findings will be industrialization, we choose the sucrose density gradient centrifugation as purified method and study the postorder work of purified virus-containing fluids.
采用蔗糖梯度离心方法提取对虾的白斑综合症病毒(WSSV)，用荧光素对病毒进行标记。
White Spot Syndrome Virus (WSSV) was purified by sucrose density gradient centrifugation, and then labeled by FLUOS.
用改良胶原酶消化技术，聚蔗糖密度梯度离心及手法挑拾方法从大鼠胰腺成功地分离出完整的胰岛。
This paper reports the successful isolation of islets of Langerhans from the rat pancreas using a modified collagenase digestion, Ficoll density gradient separation and hand-picking technique.
因此，与费劲的密度梯度离心方法相比，基于洗涤剂的细胞法分馏能简单快速地分离细胞组分（4）。
Thus, detergent-based cellular fractionation separates cellular components with greater ease and speed compared to a more laborious density centrifugation method (4).
方法：抽取兔髂骨及胫骨的骨髓液，采用密度梯度离心法结合贴壁培养法分离纯化出MSC，并增殖。
Methods: MSC were aspirated from rabbit iliac and tibias crest and purified by gradient centrifuge and adhesive culture methods.
目前国外采用较多的有荧光激活细胞分选法、磁活化细胞分选法、免疫磁珠分离法、密度梯度离心法。
Various methods of enrichment exit:fluorescent activated cell sorting, magnetic-activated cell sorting, immunomagnetic beads, density gradient.
方法：抽取兔髂骨及胫骨的骨髓液，经密度梯度离心法及塑料板贴壁培养法分离纯化出msc，并增殖。
Methods: MSC were harvested from iliac and tibias crest and obtained by using gradient centrifuge and adhesive culture methods.
经等密度梯度离心分离，从褐煤、长焰煤、气煤和贫煤四种不同变质程度煤中获得了高纯度的有机显微组分。
Coal maceral concentrates of high purity were prepared from four different rank coals by Density Gradient Centrifugation method.
结论采用全骨髓直接培养及密度梯度离心法，经3 ~5代培养后均可获得相对纯化之骨髓间充质干细胞。
Conclusion Byuse complete marrow direct culture method and density gradient method, after 3 ~ 5 passages, relative purified BMSCs can be obtained.
将腮腺炎病毒S79株培养液用中空纤维超滤器进行浓缩，经PEG沉淀后，采用蔗糖密度梯度离心法纯化抗原；
The tissue culture of mumps virus S79 strain was purified by ultrafiltration on hollow fiber, PEG precipitation and density gradient ultracentrifugation.
方法：密度梯度离心法分离44例CHF患者和20例正常人PBMC，酶消化和差速贴壁法分离乳鼠心肌细胞。
Meathod: The experiment adopted density gradient centrifuging to separate PBMC of 44 patients with CHF and 20 normal person and enzyme digestion to separate neonatal rat cardiac myocyte.
方法：取健康新西兰幼兔骨髓，密度梯度离心法分离骨髓间充质干细胞并进行体外扩增培养、成骨诱导分化及鉴定。
METHODS: Bone marrow was obtained from healthy New Zealand rabbits, by using density gradient centrifugation BMSCs were isolated and were then cultured in vitro.
结果：通过标志酶活力的监测，梯度离心后线粒体纯提高近12倍，线粒体蛋白质在双向凝胶电泳中得到很好的显现。
Results: the increase in purity of mitochondria was found to be 12 times by density gradient centrifugation. Mitochondrial proteins were displayed well in the 2 -...
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