这个数字接近总的重新平衡时间，包括负梯度洗脱。
This number is close to the total regeneration time, including the negative gradient.
预测了台阶梯度洗脱条件下待分离物质的保留体积。
The retention volume of targeted compounds was predicted in the step gradient elution conditions.
方法采用梯度洗脱高效液相色谱法分析丹参乙醇提取物。
METHODS Gradient elution HPLC was used to analyze the alcohol extracts of Radix Salviae Miltiorrhizae.
最后，利用梯度洗脱方法将大豆异黄酮由树脂柱上洗脱下来。
At last, the isoflavone is eluted with gradient aqueous alcohol.
目的建立测定注射用别嘌醇钠有关物质的高效液相梯度洗脱法。
Objective to establish a reversed phase high performance liquid chromatography gradient elution method for determination of the related substances in allopurinol sodium for injection.
结论高效液相梯度洗脱法可用于测定注射用别嘌醇钠中的有关物质。
Conclusions the reversed phase high performance liquid chromatography gradient elution method is simple and accurate for the determination of the related substances in allopurinol sodium injection.
方法利用高效液相色谱采用梯度洗脱测定疏血通注射液中的氨基酸。
METHODS Amino acid and peptides in Shuxuetong injection were determined with gradient elution.
低分子量蛋白质通过C18吸附剂吸附梯度洗脱处理血清进行富集。
Low-molecular-weight protein (LMWP) was enriched by C18 absorbent binding and elution.
采用氨基色谱柱，流动相为0.1%三氟乙酸-乙腈(梯度洗脱)。
NH2 column was used with the mobile phase of 0.1% trifluoroacetic acid-acetonitrile by gradient elution.
在等度及梯度洗脱条件下，非对映异构体辛可宁与辛可尼丁被完全分离。
Diastereomers of cinchonine and cinchonidine were fully separated both under isocratic and gradient elutions on the chiral monolithic column.
结果以氯化铵作洗脱剂进行梯度洗脱，成功的将l亮氨酸与l缬氨酸分离。
Ammonium chloride is a better eluant, and L-leucine and L-valine can be separated successfully by gradient elution.
方法：用梯度洗脱法测定清火栀麦片中穿心莲内酯和脱水穿心莲内酯的含量。
METHODS Gradient elution method was used to determine the content of andrographolide and dehydroandrographolide in Qinghuo Zhimai tablets.
本文应用反相高效液相色谱线性梯度洗脱法对保健品中的腺苷进行分离腺苷测定。
The adenosine in functional food was determined by reversed-phase high performance liquid chromatography and linearity gradient elution in this paper.
目的用反相高效液相色谱梯度洗脱法测定麻黄及其复方制剂中麻黄类生物碱的含量。
Objective To determine simultaneously the contents of ephedrine and pseudoephedrine in ephedra herb and its compound preparations by high-performance liquid chromatography.
继用氯仿-四氢呋喃-水(500：500：8)为流动相，梯度洗脱其他强保留组分。
Chloroform - tetrahydrofuran - water (500:500:8) was used as mobile phase for the gradient elution of other strongly retained substances.
梯度洗脱：仪器的配置可能会影响到方法所描述的分离度、保留时间以及相对保留时间。
Gradient elution: the configuration of the equipment employed may significantly alter the resolution, retention time and relative retentions described in the method.
基于线性溶剂强度模型，应用特征线分析的方法求解梯度洗脱模式下的理想液相色谱模型。
The ideal model of liquid chromatography in gradient elution was solved by using the characteristic approach on the assumption of a linear solvent strength model.
方法：硅胶柱色谱法，乙酸乙酯-石油醚梯度洗脱，自何首乌中提取分离二苯乙烯苷单体成分。
Methods: (1) to separate stilbene glycoside by using silica gel column chromatography with gradient elution consisting of ethyl acetate and petroleum ether.
方法采用RP -HPLC法，梯度洗脱，研究决明子饮片的指纹图谱，并作相似度比较分析。
MethodsChromatographic fingerprint of Semen Cassiae was investigated by RP-HPLC and the gradient elution mode was applied to chromatographic separation.
方法：采用梯度洗脱的方法进行色谱分离，对各色谱峰进行数据处理，得出红花黄色素的指纹图谱。
Methods: The gradient elution mode was applied in chromatographic separation and data were analyzed to develop HPLC fingerprint analysis of Safflor Yellow.
方法：（1）以硅胶柱色谱法，乙酸乙酯-石油醚梯度洗脱，自何首乌中提取分离二苯乙烯苷单体成分。
Methods: (1)To separate stilbene glycoside by using silica gel column chromatography with gradient elution consisting of ethyl acetate and petroleum ether.
采用RP-HPLC梯度洗脱方法，建立了广炎灵注射剂半成品指纹图谱，该方法简单、稳定、使用方便。
The fingerprint of GUANGYANLING injection substance was set up with RP-HPLC, gradation technology. This method is easy, stable and convenient.
用初始流动相乙腈-水(40：6 0)洗脱至主峰出峰保留时间的2.3倍时开始进行梯度洗脱比较合理。
The gradient elution is reasonable when the retention time is 2.3 times after the appearance of prominent peak with initial mobile phase acetonitrile-water (40:60).
对制备色谱的洗脱方式、洗脱剂组成、浓度、洗脱速度等参数进行优化，提出并应用了流动相速度梯度洗脱方法。
The elution mode, composition of the mobile phase, content of the organic acid and the flow-rate of the mobile phase were optimized and thus a new flow rate gradient was proposed.
方法采用HPLC法，以君药红参中人参皂苷为检测指标，采用梯度洗脱的方法，控制其中红参主要成分的色谱峰。
Method The main ingredient of the injection, Red Ginseng, was determined by HPLC and used binary solvent-delivery gradient elution to control the main component in Red Ginseng.
方法以甲醇-水为流动相，采用HPLC梯度洗脱法，测定了雷公藤药材的根、枝、叶中雷公藤甲素在不同存贮期的含量。
METHOD HPLC gradient elution method was used with methanol-water as the mobile phase to determine the contents of triptolide in its root, branch and leaf at different periods of its storage.
你引用的规则是用于覆盖全范围梯度洗脱的（例如，有机溶剂从5改变到100%），并且所指的体积是以初始条件来重新平衡。
The rule that you quoted is intended to cover a full-range gradient (for example, 5–100% organic solvent) and refers to the amount of volume to reequilibrate at the initial conditions.
方法：利用RPHPLC ，采用线性梯度洗脱等进行指纹图谱的测定，并应用LC/MS技术指认指纹图谱中的主要色谱峰。
Method: RP- HPLC, linear gradient elution, LC/MS, etc. were used to determine the fingerprint and identify the main pea ks in the HPLC fingerprint.
方法：利用RPHPLC ，采用线性梯度洗脱等进行指纹图谱的测定，并应用LC/MS技术指认指纹图谱中的主要色谱峰。
Method: RP- HPLC, linear gradient elution, LC/MS, etc. were used to determine the fingerprint and identify the main pea ks in the HPLC fingerprint.
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