结果构建的EBO G87和EBO WT重组载体经内切酶双酶切鉴定及核苷酸序列测定证实。
Results The constructed vectors of EBO-WT and EBO-G87 were identified by restriction enzyme digestion and nucleotide sequencing.
对分离到的HEV71阳性分离株进行VP1编码区基因扩增，核苷酸序列测定和同源进化分析。
And identified HEV71 isolates were performed by gene amplification of VP1 coding region, nucleotide sequencing and homology analysis of evolution.
部分扩增产物t A克隆后测定核苷酸序列。
Partial amplification products were sequenced after T-A cloning.
HGV高度保守区5＇UTR的分子克隆与核苷酸序列的测定对开展HGV感染的基因诊断具有重要意义。
The sequencing of highly conserved 5' UTR of the Chinese HGV strain has important implication for the development of genetic diagnostic assays.
利用基因组核苷酸全序列测定、电镜观察、血清学测定、传播介体传毒试验等方法对山东省田间发生的玉米病毒病进行了病原鉴定。
Identification on pathogens of maize viral diseases in Shandong province was conducted by whole genomic sequencing, electron microscope observation, serological analysis and vector transmitting test.
其中碳末端45 0个核苷酸的序列测定和分析提示，该7株病毒为麻疹野病毒H1基因型。
Sequence analysis of 450bp of C-terminus of the N gene indicated that all of the 7 isolates were H1 genotype.
其中碳末端45 0个核苷酸的序列测定和分析提示，该7株病毒为麻疹野病毒H1基因型。
Sequence analysis of 450bp of C-terminus of the N gene indicated that all of the 7 isolates were H1 genotype.
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