部分扩增产物t A克隆后测定核苷酸序列。

Partial amplification products were sequenced after T-A cloning.

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在FMDV的核苷酸序列相对保守区设计上下游引物FM1/FM4，扩增病毒的基因片段。

Then capture FMDV from clinical material with sterilized Eppendrof tubes of coating type-specific IgG, Amplify the conserved viral sequences by common primers (FM1/FM4).

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对分离到的HEV71阳性分离株进行VP1编码区基因扩增，核苷酸序列测定和同源进化分析。

And identified HEV71 isolates were performed by gene amplification of VP1 coding region, nucleotide sequencing and homology analysis of evolution.

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对分离到的HEV71阳性分离株进行VP1编码区基因扩增，核苷酸序列测定和同源进化分析。

And identified HEV71 isolates were performed by gene amplification of VP1 coding region, nucleotide sequencing and homology analysis of evolution.

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