作者就无透明带核移植技术和单个胚胎培养系统作一介绍。
This paper introduces the methods of zona-free SCNT and the single embryo culture system.
目前，世界上通过体细胞核移植技术已经产生了许多的克隆动物。
So far cloned animals have been produced in many species by somatic nuclear transfer.
但是核重编程仍是核移植技术的瓶颈，制约了重构胚胎干细胞的研究。
However, nuclear reprogramming, which stands in the way of reconstructed embryo stem cell research, is still a bottleneck in the procedure of nuclear transfer.
逐步成熟的核移植技术，必将给畜牧业和生物学、医学等研究领域带来深远的影响。
This technology would have brought great influences on livestock husbandry, biological, and medical research.
用体细胞核移植技术生产转基因动物是目前一种最新、最有效的生产转基因动物的方法，而转基因体细胞系的建立是用这一方法生产转基因动物的基础。
Somatic cells nuclear transfer offers an efficient and practical method of producing transgenic animals, and the establishment of transgenic somatic cell lines are the basic of this method.
治疗性克隆是通过核移植和干细胞技术为病人提供所需更换的细胞、组织乃至器官。
Therapeutic cloning based on application of nuclear transfer and stem cell technology is targeted to provide an unlimited supply of compatible cells, tissue and organs for human transplantation.
猪的核移植研究意义重大，应用价值颇高，是当今生物技术的热点研究之一。
The research of porcine somatic nuclear transfer has huge scientific significance and applied value, which is one of hot point of biotechnology.
将胚胎分割技术与细胞核移植、临床诊断等相结合，将具有更为广阔的应用前景。
There will be wider application prospect if we combine the embryo bisection with new biotechniques such as cell nuclear transfer, diagnosis before child birth, and so on.
核移植克隆猪技术的出现为生产转基因猪提供了一种有效的方法，并且是目前生产基因打靶猪的惟一方法。
Pig cloning provides an efficient method for producing transgenic pigs, further more, it is the only approach which has been used to produce gene-targeted pigs so far.
核移植克隆猪技术的出现为生产转基因猪提供了一种有效的方法，并且是目前生产基因打靶猪的惟一方法。
Pig cloning provides an efficient method for producing transgenic pigs, further more, it is the only approach which has been used to produce gene-targeted pigs so far.
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