方法4例因献血感染HCV病人的系列血标本,用特异性引物扩增5'-NCR和核心区基因并进行测序。
Methods Serial sera were collected from 4 plasma-donors with HCV infection. 5' - NCR and core gene were amplified, sequenced and analyzed using software.
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其核心是RMAPD引物的有效性问题。
The core question of RMAPD is validity of the primers used.
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