方法根据APP的基因序列，设计并合成引物和荧光标记探针。
Methods According to the specific sequence of APP genes, the primers and the fluorogenic probe were designed and synthesized.
细菌生理群的研究，可采用快速多点法、复制技术、放射性自显影及标记探针法。
Rapid multipoint method, replica technique, autoradiography signature probe are used in the study on physiological groups of bacteria.
并分析了荧光标记探针的发展趋势，指出了理想的荧光标记探针应该具备的特征。
Its research development trend is analyzed. Finally, necessary conditions to obtain the ideal fluorescent label probe are pointed out.
目的探讨PCR -荧光双标记探针杂交定量结核杆菌技术对肺结核的诊断价值并进行卫生经济学评价。
Objective To probe the diagnostic value and health economic evaluation of PCR-fluorogenic hybridization assay in pulmonary tuberculosis.
经生物素标记探针杂交确认RANTES基因的整合，用免疫斑点印迹法对重组基因表达水平进行定量测定。
The integration of RANTES gene was confirmed by hybridization with biotin-labelled probes and the recombinant gene expression level was quantitatively detected by immunodot blotting.
优化了基于纳米金标记探针的目视化检测方法，将其用于检测从阳性血清中提取的乙肝病毒(HBV)基因，并与基于荧光素异硫氰酸酯(FITC)标记探针的荧光检测方法进行了比较。
Visual method based on nanogold-supported probes was optimized and used to detect HBV extracted from positive serum samples, which was compared with fluorescence method based on FITC-labeled probes.
与Fodor博士不同的是，斯坦福的研究者利用被称为“探针”的合成前DNA链，然后将其利用机器人放置或“标记”在载玻片之上。
Unlike Dr Fodor's method, the Stanford researchers used pre-synthesised DNA strands, called probes, that were placed or “spotted” on the slide by a robot.
研究人员已经研制出一种比绿色荧光蛋白小的蓝色荧光探针，它更容易标记活细胞中肽融合重组蛋白。
Researchers have engineered a blue fluorescent probe that is smaller than GFP and will improve the labeling of peptide-fused recombinant proteins in living cells.
研究组通过最初的方法使用更小的探针有效地标记肌动蛋白以及参与细胞分裂，结构，运动和信息传导的分子。
The team used these much smaller probes with the PRIME method to effectively tag actin, a molecule involved in cell division, structure, motility, and communication.
该研究小组还利用新的探针开发了一种新的蛋白标记方法，称为探针的酶介导法(PRIME)。
The group also developed a new protein labeling method, called probe incorporation mediated by enzymes (PRIME), which utilizes the new probes.
寡核苷酸探针的标记非常重要。
需要使用包括聚合酶链反应（PCR），琼脂糖凝胶电泳，探针的标记与检测等生物工程技术。
This technique employs standard operations of biomedical engineering, such as polymerase chain reaction(PCR), agarose gel electrophoresis, labeling and detecting of probe.
相对较大的荧光蛋白探针会干扰肌动蛋白的功能和运动，但是通过小探针标记的肌动蛋白分子能够通过细胞甚至进入核心。
The relatively larger GFP interferes with the function and mobility of actin, but actin molecules tagged with the smaller PRIME probes were able to move throughout the cell and even enter the nucleus.
DNA微集阵列技术的关键是探针的制备与样品的标记、杂交。
The key points of the DNA microarray technology include probe preparation, sample labeling and hybridization.
本文从纳米粒子的分类、制备、标记和检测原理等方面对纳米粒子标记DNA探针的制备和应用进行了分析与评述。
In this paper, the classification, manufacture, labelled methods and principle of detection of nanoparticle tagging DNA probe and its application in gene detection were described and reviewed.
纳米粒子标记DNA探针的研究是纳米技术、基因组学、DNA检测等学科交叉的研究热点。
Nanoparticle tagging DNA probe, which combines of nano-technology, gene project and DNA detection, is the focus of recent research field.
对扫描探针显微镜(SPM)仪器漂移的定量测量的几种方法进行探讨，提出应用二维零位标记进行漂移测量。
Several quantitative drift measurement techniques for scanning probe microscopy (SPM) were introduced. A new method using two-dimensional zero-reference masks was proposed to measure the SPM drift.
另一条为检测探针，3端标记生物素。
本发明不仅可利用该无毒基因的标记作探针，而且为进一步通过物理作图法克隆该无毒基因奠定了基础。
The invention can not only make use of the nontoxic gene marker as gene probe, but also set foundations for further clone of the gene through physical mapping.
应用生物素标记HBVDNA（乙肝病毒脱氧核糖核酸）作探针，对129例肝病患者肝组织作原位杂交研究。
A biotin-labelled DNA specific for hepatitis B virus (HBV) nucleo-tide sequences was hybridized in situ to liver tissue of 129 cases with liver diseases.
该文就量子点探针的表面修饰和功能化及其标记技术的研究进展进行了阐述。
This article briefly discusses the recent progresses on fluorescent QDs probes and their biological labeling including their surface modification and functionalization.
方法：采用AO探针标记，激光共聚焦技术观测槟榔碱对骨髓细胞内DNA、RNA 的影响；采用流式细胞仪测定槟榔碱对骨髓细胞周期的影响。
Methods:Laser Confocal Microscope was adopted to measure DNA and RNA of the marrow cells marked by AO probe.
第二部分：利用金纳米粒子标记的DNA适配子为探针测定凝血酶的共振光散射分析。
Part two: Study on the Resonance Light Scattering analysis to determine thrombin by using gold nanoparticle-labeled DNA aptamer as probe.
本文以藻红蛋白为荧光探针，探讨了藻红蛋白荧光标记技术及荧光探针在烟草花叶病毒免疫检测中的应用效果。
The fluorescence labeling technique of phycoerythrin and application for the immunoassay of Tobacco mosaic Virus were studied in this research using it as a labeling probe.
方法：采用电子自旋共振（ESR）、氧自旋探针和自旋标记等技术方法测定。
Methods: Electron spin resonance (ESR) and the new techniques of spin labeling and oxygen spin probe were used in the study.
本研究采用地高辛标记的RNA探针，用原位杂交的方法研究了大鼠胃底腺中胃蛋白酶产生细胞的个体发育。
In this study, the ontogeny of pepsinogen producing cells in rat fundic glands was studied by in situ hybridization using a digoxigenin-labeled RNA probe.
方法P CR -ELISA以地高辛标记端粒重复片段特异的探针与PCR变性产物杂交，通过ELISA检测端粒酶活性。
Methods We used telomerase PCR-ELISA an assay in which a digoxigenin labeled telomerase repeat-specific probe is hybridized to the PCR product detecting with ELISA.
简述了荧光染料探针分子用于变异细胞组织的标记与识别的研究新进展。
The progress of research on labeling of aberrant cell tissue by fluorescent probe or by connecting with biologically active carrier is reviewed.
被标记的是样本，而非探针。
被标记的是样本，而非探针。
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