采用薄层色谱法（TLC）确定柱层析的洗脱剂，并检测柱层析的分离效果，采用红外光谱法（IR）进行结构分析。
The eluant was selected by TLC and TLC was also used to identify the effect of the TLC separation, and the molecular constructures were characterized by IR.
用离子交换柱层析法分离，用反相高效液相色谱法进一步纯化，经聚丙烯酰胺凝胶电泳分析确定相对分子质量。
Methods The peptide was separated by ion exchange column chromatography and further purified by reverse phase HPLC, and the molecular weight was determined by tricine gel electro phoresis.
用离子交换柱层析法分离，用反相高效液相色谱法进一步纯化，经聚丙烯酰胺凝胶电泳分析确定相对分子质量。
Methods The peptide was separated by ion exchange column chromatography and further purified by reverse phase HPLC, and the molecular weight was determined by tricine gel electro phoresis.
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