运用正交试验设计理论,对高产L-乳酸突变株NAF-032的最佳摇瓶发酵条件进行了研究,确定了最佳的发酵培养基和发酵条件。
The optimal fermentation medium and conditions of mutant strain NAF-032 with a higher and stable yield of L-lactic acid were studied by orthogonal design theory.
结果显示,有一个突变株不能分化异形胞;三个突变株可以形成异形胞,但在缺氮的条件下仍不能维持生长;
The result showed that one mutant was unable to differentiate heterocyst, three formed heterocysts but still could not maintain normal growth, four growed slowly with heterocyst differentiation.
同时,改变发酵液中不同氮源和碳源,对突变株的发酵条件进行了比较研究。
Also the fermentation conditions were studied with different carbon sources and nitrogen sources.
本文还对突变株60d7z53发酵产酶培养基和培养条件进行了优化。
The optimal culture medium and conditions of cellulase production on the mutant 60d7z53 were carried out.
对纤溶酶产生菌的突变株进行发酵条件优化;
The optimal fermentation conditions for producing fibrinolytic enzyme were determined;
对高活性突变株m 14的细胞转化和发酵转化条件进行优化。
The conditions of fermentable and enzymatic conversion of M14 were optimized.
采用新型常压室温等离子体射流诱变产油酵母,结合快速突变产油酵母操作条件及基于96孔板的高通量筛选手段,获得了一系列增殖速度和产油量发生变化的突变株。
To obtain oleaginous yeast mutants with improved lipid production and growth rates, an atmospheric and room temperature plasma(ARTP) jet was used with a 96-well plate for high throughput screening.
采用新型常压室温等离子体射流诱变产油酵母,结合快速突变产油酵母操作条件及基于96孔板的高通量筛选手段,获得了一系列增殖速度和产油量发生变化的突变株。
To obtain oleaginous yeast mutants with improved lipid production and growth rates, an atmospheric and room temperature plasma(ARTP) jet was used with a 96-well plate for high throughput screening.
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