介绍了杆状病毒表达系统及其优点。
Baculovirus expressed system and its advantages were introduced.
杆状病毒DNA解旋酶是病毒复制所必需的。
DNA helicases are essential for replication of baculoviruses.
同时也介绍了杆状病毒基因组研究的新结果。
Recent research progress of baculovirus genomics was introduced as well.
文中综述了杆状病毒在以上方面应用的最新进展。
The advances in application of Baculoviruses are reviewed in this paper.
第一章对杆状病毒及泛素系统的研究作了简要的论述。
Chapter One is a brief review on recent advances on baculovirology and ubiquitin system.
该病毒作为载体，应用在杆状病毒-昆虫真核生物表达系统。
It is applied as a carrier in expression system of granulosis virus insect eucaryotic biology.
经同源重组后，以终点稀释和斑点杂交法筛选重组杆状病毒；
After co-transfection of recombinant plasmid and wild type AcNPV DNA, the rAcNPV was confirmed by the end point dilution assay and dot blot.
目的探讨人源抗甲型肝炎病毒全抗体分子在杆状病毒中的表达。
Objective To develop human recombinant neutralizing IgG monoclonal antibodies to hepatitis A virus (HAV) by baculovirus expression system.
目的利用杆状病毒表达载体系统制备重组乙型肝炎病毒核心蛋白。
Objective To produce recombinant HBcAg in insect cells by Baculovirus expression vector system.
目的探讨重组杆状病毒作为哺乳动物基因转导载体的可行性及其转导特点。
Objective to study the feasibility and the characteristics of recombinant baculovirus as mammalian gene transfer vector.
应用透射电镜和超薄切片技术，对长毛对虾和斑节对虾的杆状病毒病进行研究。
With transmission electron microscope and technique of ultrathin section, we studied the baculovirus diseases on Penaeus Penicillatus Alcock and Penaeus Monodon Fabricus.
目的建立昆虫杆状病毒表达系统表达人II型CD 74胞外端片段的技术平台。
Objective to establish an expression system of human II CD74 extracellular fragment by insect baculovirus vector expression system in insect cells.
人工感染试验结果证明，对虾暴发性流行病的病原为皮下及造血组织坏死杆状病毒。
The results of artificial infection demonstrated that the pathogen of EEDS in 1993 was hypodermal and hematopoietic necrosis baculovirus.
结果表明，造成养殖对虾爆发性病害的主要原因是对虾杆状病毒与细菌交叉感染所致。
The result of the observation shows that cross-infection of the penaeid baculovirus and germ into penaeid cell was the main pathogeny causing the unexpected virus disease of penaeid shrimp.
杆状病毒的分子生物学，基因功能、复制机制，及病毒与不同宿主细胞的相互作用关系。
The molecular biology, gene function and replication of baculovirus, and its interaction with various hosts cells.
为了建立配套诊断方法，通过杆状病毒表达系统表达了N3亚型禽流感病毒的NA蛋白。
To set up companion diagnosis method for it, the NA protein of N3 subtype AIV was expressed in baculovirus expression systems.
然后使用重组杆状病毒技术使这些S1亚单位在昆虫细胞和昆虫幼虫中实现了高水平表达。
The modified S1 subunits were abundantly expressed by recombinant autographa californica nuclear polyhedrosis viruses in insect cells and larva.
这些病害包括甘蔗温性嵌纹病、甘蔗黄叶综合症、甘蔗干梢腐病、甘蔗杆状病毒病和白条病。
These diseases include Sugarcane MildMosaic Virus, Sugarcane Yellowleaf Syndrome, Dry Top Rot, Sugarcane BacilliformVirus and leaf Scald.
相较于前两年，今 年养成场并未检出白点症、草虾杆状病毒症、黄头病毒症及毛利病毒症。
Compared to results in the previous two years, White Spot Syndrome (WSS), Monodon Baculovirus Disease, Yellowhead Disease and Mourilyan Virus Disease were not found this year.
结论：杆状病毒载体能够介导HHV - 8肿瘤转化基因k12编码的蛋白在昆虫细胞中表达。
Conclusion: Baculovirus transfer vector may mediate the coding protein of HHV-8 K12 gene expressed in insect cells.
杆状病毒载体将在未来药物研发、疫苗生产、基因治疗、重组杆状病毒杀虫剂等领域得到广泛应用。
The insect baculovirus, in the new century, will be applied in drug research and development, production of vaccines, gene therapy and recombinant baculovirus insecticides.
结论杆状病毒表达系统能成功表达有活性的CYP2B6重组酶，可用来进一步研究在药物代谢中的作用。
CONCLUSION Active recombinant human CYP2B6 obtained using baculovirus expression system will facilitate further examination of the role of this enzyme in drug metabolism.
系统分析表明，杆状病毒IAP基因可能是病毒与鳞翅目昆虫在长期的进化过程中从宿主基因组中获得的。
Phylogenetic analysis of IAP genes and lots of evidence sppport the origin of this viral gene by capture of a host gene early in the evolution of Lepidoptera.
水稻东格鲁病是由水稻东格鲁杆状病毒（RTBV）和水稻东格鲁球状病毒（RTSV）复合感染引起的。
Rice tungro is caused by Rice tungro bacilliform virus (RTBV) and Rice tungro spherical virus (RTSV) in a composite manner.
另外，杆状病毒是非复制型载体，能高效表达目的蛋白，其作为基因转移载体在基因治疗中乙显示出良好的应用前景。
Additionally, baculovirus has been a new gene transfer vector used in gene therapy due to its advantages of the extremely high expression of foreign genes and being non-reproduce vector.
近年来杆状病毒介导的昆虫表达系统得到了广泛地应用，本课题通过该系统表达CYP2B6重组酶，并进行活性鉴定。
In this study, CYP2B6 recombinant enzyme was obtained using baculovirus expression system and its catalytic activity was measured using bupropion as probe.
间接免疫荧光、ELISA和免疫印迹检测证明，含SO.7片段的三种重组杆状病毒均在昆虫细胞中表达出相应的活性蛋白。
By IFA, ELISA and Western blot experiments, it was proved all the recombinant baculoviruses containing SO. 7 segment could express biologically active proteins in insect cells.
综述了与杆状病毒DNA复制相关基因的研究进展。杆状病毒表达系统是最重要的四大基因工程表达系统之一，杆状病毒还具有作为生物杀虫剂的潜能。
Baculovirus expression system (BES) is one of the most important expression systems. Baculovirus also has potential ability as pesticide. DNA replication is the central step in its life cycle.
结论：利用杆状病毒表达系统，成功地表达同时具有核蛋白及糖蛋白g 2生物学活性的融合蛋白g2s0 . 7，为进一步研究其免疫学特性奠定了基础。
CONCLUSION: the successful expression of fusion protein G2S0.7 with biological activity in insect cells, which lays the foundation for further research on its immunological characteristic.
结论：利用杆状病毒表达系统，成功地表达同时具有核蛋白及糖蛋白g 2生物学活性的融合蛋白g2s0 . 7，为进一步研究其免疫学特性奠定了基础。
CONCLUSION: the successful expression of fusion protein G2S0.7 with biological activity in insect cells, which lays the foundation for further research on its immunological characteristic.
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